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Inhibitory Effect of Ascorbic Acid on the Proliferation and Invasion of Hepatoma Cells in Culture

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Abstract

Effect of ascorbic acid (AsA) on the proliferation and invasion of rat ascites hepatoma AH109A cells was investigated by measuring [3H]thymidine incorporation into acid-insoluble fraction of the cells and by co-culturing the hepatoma cells with rat mesentery-derived mesothelial cells, respectively. AsA suppressed the invasion of AH109A cells in a dose-dependent manner at concentrations of 62.5–500 μM, while it inhibited the proliferation of the cells at higher concentrations of 250 and 500 μM. Hepatoma cells previously cultured with hypoxanthine (HX) and xanthine oxidase (XO) or with hydrogen peroxide showed increased invasive activities. AsA suppressed the reactive oxygen species-potentiated invasive capacity by simultaneously treating AH109A cells with AsA, HX and XO or with AsA and hydrogen peroxide. Furthermore, AsA reduced the intracellular peroxide levels in AH109A cells. These results suggest that the antioxidative property of AsA may be involved in its anti-invasive action on hepatoma cells.

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Abbreviations

AsA:

ascorbic acid

CS:

calf serum

HX:

hypoxanthine

M-cells:

mesothelial cells

MEM:

Eagle’s minimum essential medium

ROS:

reactive oxygen species

XO:

xanthine oxidase

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Authors and Affiliations

  1. Department of Applied Biological Science, Tokyo Noko University, Saiwai-cho 3-5-8, Fuchu, Tokyo, 183-8509, Japan

    Nobuhiro Hirakawa, Yutaka Miura & Kazumi Yagasaki

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  1. Nobuhiro Hirakawa
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  2. Yutaka Miura
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  3. Kazumi Yagasaki
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Correspondence to Kazumi Yagasaki.

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Hirakawa, N., Miura, Y. & Yagasaki, K. Inhibitory Effect of Ascorbic Acid on the Proliferation and Invasion of Hepatoma Cells in Culture. Cytotechnology 47, 133–138 (2005). https://doi.org/10.1007/s10616-005-3750-y

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  • DOI: https://doi.org/10.1007/s10616-005-3750-y

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