Abstract
Resveratrol, present in grapes and other plants, is a polyphenolic compound with strong antioxidative activity. In our previous studies, we found that reactive oxygen species (ROS) accelerated the invasive capacity of a rat ascites hepatoma cell line of AH109A in culture and that resveratrol and resveratrol-loaded rat sera suppressed the ROS-potentiated invasion of the hepatoma cells. To study mechanisms by which resveratrol and itsin vivo metabolite(s) suppress the invasion, we estimated intracellular peroxide level and expression of hepatocyte growth factor (HGF), a known cell motility factor, in AH109A cells. Exogenously added ROS promoted the intracellular peroxide level and the expression of HGF. Resveratrol and resveratrol-loaded rat sera canceled the rise in the peroxide level and HGF expression in ROS-stimulated tumor cells. These results suggest an involvement of the antioxidative property of resveratrol and sera from rats orally given resveratrol in their suppressive effects on ROS-potentiated invasion of AH109A cells.
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Abbreviations
- AH109A:
-
a rat ascites hepatoma cell line of AH109A
- CMC:
-
carboxymethyl cellulose sodium salt
- CS:
-
calf serum
- DCFH-DA:
-
2’,7’-dichlorofluorescin diacetate
- DMSO:
-
dimethyl sulfoxide
- ELISA:
-
enzyme-linked immunosorbent assay
- HGF:
-
hepatocyte growth factor
- HX:
-
hypoxanthine
- IC50 :
-
50% inhibitory concentration
- M-cells:
-
rat mesentery-derived mesothelial cells
- MEM:
-
Eagle’s minimum essential medium
- PBS:
-
phosphate buffered saline
- ROS:
-
reactive oxygen species
- RS:
-
rat serum
- XO:
-
xanthine oxidase
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Miura, D., Miura, Y. & Yagasaki, K. Resveratrol inhibits hepatoma cell invasion by suppressing gene expression of hepatocyte growth factor via its reactive oxygen species-scavenging property. Clin Exp Metastasis 21, 445–451 (2004). https://doi.org/10.1007/s10585-004-2698-1
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DOI: https://doi.org/10.1007/s10585-004-2698-1