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SNAI1 is Involved in the Proliferation and Migration of Glioblastoma Cells

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Abstract

Glioblastoma is the most common type of astrocytoma in the brain. Due to its high invasiveness and chemoresistance, patients with advanced stage of glioblastoma have a poor prognosis. SNAI1, an important regulator of epithelial-mesenchymal transition, has been associated with metastasis in various carcinoma cells. However, its roles in glioblastoma cells have been poorly characterized. To examine roles of SNAI1 in glioblastoma cells, we knockdowned SNAI1 expression using siRNA. SNAI1 siRNA increased the expression level of E-cadherin and decreased that of vimentin. In the water-soluble tetrazolium salt (WST-1) assay, SNAI1 siRNA inhibited the proliferation of U87-MG and GBM05 glioblastoma cells. Moreover, in the Boyden chamber assay and Matrigel invasion assay, SNAI1 siRNA inhibited serum-induced migration and invasion of glioblastoma cells. These results suggested that SNAI1 is involved in the proliferation and migration of glioblastoma cells.

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Acknowledgments

This study was supported by Medical Research Institute Grant (2006-12), Pusan National University and a grant from the National R&D Program for Cancer Control, Ministry for Health, Welfare and Family Affairs, Republic of Korea (0920050).

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Authors and Affiliations

  1. Department of Anatomy, School of Medicine, Pusan National University, Beomeo-Ri, Mulgeum-Eup, Yangsan, 626-870, South Korea

    Sung-Pil Han, Myoung-Eun Han, Hey-Eun Sim, Ki-Sun Kim, Sik Yoon, Sun-Yong Baek, Bong-Seon Kim & Sae-Ock Oh

  2. Medical Research Center for Ischemic Tissue Regeneration, Pusan National University, Beomeo-Ri, Mulgeum-Eup, Yangsan, 626-870, South Korea

    Sung-Pil Han, Myoung-Eun Han, Hey-Eun Sim & Sae-Ock Oh

  3. School of Medicine, Pusan National University, Beomeo-Ri, Mulgeum-Eup, Yangsan, 626-870, South Korea

    Ji-Hoon Kim

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  1. Sung-Pil Han
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Correspondence to Sae-Ock Oh.

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Sung-Pil Han and Ji-Hoon Kim equally contributed to this work.

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Han, SP., Kim, JH., Han, ME. et al. SNAI1 is Involved in the Proliferation and Migration of Glioblastoma Cells. Cell Mol Neurobiol 31, 489–496 (2011). https://doi.org/10.1007/s10571-010-9643-4

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  • DOI: https://doi.org/10.1007/s10571-010-9643-4

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