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Identification of the role of Smad interacting protein 1 (SIP1) in glioma

  • Laboratory Investigation - Human/Animal Tissue
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Abstract

Glioma is an extremely aggressive and lethal form of brain cancer. Despite recent advances in diagnostics and treatments, prognosis for advanced patients suffering from these diseases remains poor. Therefore, identification of new therapeutic targets for glioma is of significant importance. In this study, we identified the important role of Smad interacting protein 1 (SIP1; also known as ZEB2) in glioma. We firstly found that SIP1 expression was high in four tumorigenic glioma cell lines but low in two nontumorigenic glioma cell lines. By knockdown or overexpression assay, we discovered that knockdown of SIP1 expression statistically significantly inhibited cell migration and invasion of tumorigenic glioma cells, while overexpression of SIP1 promoted cell migration and invasion of nontumorigenic glioma cells. SIP1 knockdown inhibits and overexpression promotes glioma cell clonogenicity in vitro. Further studies identified that SIP1 overexpression inhibits expression of E-cadherin and enhances expression of mesenchymal proteins such as fibronectin and vimentin. This study supports the rationale for developing SIP1 as a potential therapeutic and diagnostic target for gliomas.

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Acknowledgments

This work was supported by the Anhui National Science Foundation (2004kj341).

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Authors and Affiliations

  1. Integrative Laboratory, The University of Hong Kong, Pokfulam, Hong Kong, China

    Microgene Xia

  2. Yijishan Hospital, Wannan Medical College, Wuhu, 241001, China

    Minghua Hu, Jun Wang & Yajun Xu

  3. Department of Internal Medicine, Henan Tumor Hospital, Zhengzhou, 450008, China

    Xiaobing Chen

  4. Department of Internal Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510080, China

    Yuedong Ma & Lei Su

  5. Department of Surgery, Yijishan Hospital of Wannan Medical College, Anhui, 241001, China

    Minghua Hu

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  1. Microgene Xia
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  2. Minghua Hu
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  3. Jun Wang
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  4. Yajun Xu
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  5. Xiaobing Chen
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  6. Yuedong Ma
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  7. Lei Su
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Corresponding authors

Correspondence to Microgene Xia or Minghua Hu.

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Microgene Xia and Minghua Hu contributed equally to this work.

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Xia, M., Hu, M., Wang, J. et al. Identification of the role of Smad interacting protein 1 (SIP1) in glioma. J Neurooncol 97, 225–232 (2010). https://doi.org/10.1007/s11060-009-0015-1

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  • DOI: https://doi.org/10.1007/s11060-009-0015-1

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