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Generation of lignin and enzymatically digestible cellulose from ethanol-based organosolv pretreatment of sugarcane bagasse

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Abstract

This work proposes a new methodology for sugarcane bagasse pretreatment with ethanol, allowing production of lignin and enzymatically digestible cellulose in a one-step fractionation process. After ethanol-based organosolv pretreatment, the enzymatic digestibility of pretreated sugarcane bagasse was greatly enhanced: based on 100 g raw material, 39.96 g glucose could be obtained during enzymatic hydrolysis, representing 91.39 % of the glucose in the raw material. Furthermore, characterization of raw material and solids pretreated at various temperatures by X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier-transform infrared (FT-IR) spectroscopy, and thermogravimetric (TG) analysis was carried out to better understand how delignification and hemicellulose removal affected subsequent enzymatic hydrolysis. Pyrolysis–gas chromatography/mass spectroscopy (Py–GC/MS) analysis of lignins recovered after ethanol-based pretreatment and enzymatic residues indicated that the two lignin fractions generated different pyrolysis products, but they were both enriched in p-hydroxyphenyl (H) units.

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Acknowledgments

This work was supported by the National Key Basic Research Program of China (no. 2013CB228101), National High Technology Research and Development Program of China (no. 2012AA101806), National Natural Science Foundation of China (no. 31270635), and fundamental research funds for the central universities (no. 2014ZP0014).

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Authors and Affiliations

  1. State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, 510640, Guangdong, People’s Republic of China

    Hongdan Zhang & Shubin Wu

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  1. Hongdan Zhang
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  2. Shubin Wu
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Correspondence to Shubin Wu.

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Zhang, H., Wu, S. Generation of lignin and enzymatically digestible cellulose from ethanol-based organosolv pretreatment of sugarcane bagasse. Cellulose 22, 2409–2418 (2015). https://doi.org/10.1007/s10570-015-0678-z

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  • DOI: https://doi.org/10.1007/s10570-015-0678-z

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