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Comparative evaluation of the action of two different endoglucanases. Part II: On a biobleached acid sulphite pulp

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Abstract

A TCF sulphite pulp, bleached at the laboratory scale with a laccase–violuric acid system and complemented with a pressurized hydrogen peroxide stage, was treated with two endoglucanases, one obtained from Paenibacillus barcinonensis (B) and the other one produced from Cerrena unicolor (F) to improve cellulose reactivity. The treated pulps were evaluated in terms of brightness, viscosity, α-cellulose, water retention value, fibre morphology, Fock solubility, NMR and carbohydrate composition of pulps and liquors. Results revealed that both endoglucanases improved cellulose reactivity, albeit in a different way; thus, B caused no scissions in the cellulose chain and no significant reduction in fibre length, whereas F decreased viscosity and shortened fibre length, leading to lower reactivity value. The liquor composition of soluble carbohydrates released by the enzymatic treatments revealed the B had a processive mode of action since short oligosaccharides, cellobiose and glucose, were obtained. F hydrolysed, from high to low concentration, cellobiose, glucose and cellotriose. Importantly, environmentally friendly dissolving pulp with 90 % Fock solubility was obtained, combining two enzymatic treatments: a laccase–mediator system and then a cellulase from P. barcinonensis (B). In order to improve the quality of final dissolving pulp, a pulp purification step was introduced before the B endoglucanase treatment. The cold caustic extraction lead to reduce the amount of hemicelluloses by 42 % with respect to biobleached pulp, but Fock solubility was also reduced. However, complementing the purification step with F treatment, reduced the amount of hemicelluloses but also improved Fock solubility by 17 %, although some presence of cellulose II was detected by NMR.

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Abbreviations

CCE:

Cold caustic extraction

CS:

Chain scission

DP:

Degree of polymerization

EG:

Endoglucanase

K_CCE:

Control treatment with buffer acetate + cold caustic extraction

L:

Laccase–mediator treatment

L_B120:

Biobleached pulp + B endoglucanase at 120 U/g

L_CCE:

Biobleached pulp + cold caustic extraction

L_CCE_B120:

Biobleached pulp + cold caustic extraction + B endoglucanase at 120 U/g

L_CCE_F12:

Biobleached pulp + cold caustic extraction + F endoglucanase at 12 U/g

L_F12:

Biobleached pulp + F endoglucanase at 12 U/g

L_K:

Biobleached pulp + control treatment with buffer acetate

PO:

Hydrogen peroxide reinforced with pressurized oxygen

TCF:

Totally chlorine free

n.d.:

Not determined

odp:

Oven dried pulp

VA:

Violuric acid

WRV:

Water retention value

13C-CP/MAS NMR:

Solid state cross polarization/magic angle spinning carbon 13 nuclear magnetic resonance

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Acknowledgments

The authors thank the "Ministerio de Economía y Competitividad" of Spain for their support in this work under the projects BIOSURFACEL CTQ2012-34109 (funding also from the "Fondo Europeo de Desarrollo Regional FEDER") and BIOPAPμFLUID CTQ2013-48995-C2-1-R. The authors are grateful to the consolidated group with the Universitat de Barcelona (UB) AGAUR 2014 SGR 534. The authors would like to thank DÖMSJO (Sweden) for providing the starting pulp, Fungal Bioproducts (Spain) for kindly supplying the F cellulase and the Department of Microbiology (University of Barcelona, Spain) for warmly providing the B cellulase.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. CELBIOTECH_Paper Engineering Research Group, Universitat Politècnica de Catalunya, BarcelonaTech, 08222, Terrassa, Spain

    Elisabet Quintana, Cristina Valls, Teresa Vidal & M. Blanca Roncero

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  1. Elisabet Quintana
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  2. Cristina Valls
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  3. Teresa Vidal
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  4. M. Blanca Roncero
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Correspondence to Elisabet Quintana or M. Blanca Roncero.

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Quintana, E., Valls, C., Vidal, T. et al. Comparative evaluation of the action of two different endoglucanases. Part II: On a biobleached acid sulphite pulp. Cellulose 22, 2081–2093 (2015). https://doi.org/10.1007/s10570-015-0631-1

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