Skip to main content
SpringerLink
Log in

Improved Catalytic Performance of Aspergillus flavus Laccase Immobilized on the Zinc Ferrite Nanoparticles

  • Published:
Catalysis Letters Aims and scope Submit manuscript

Abstract

In this study, zinc ferrite nanoparticles have been synthesized by co-precipitation method and used as carrier for the immobilization of Aspergillus flavus laccase. Identification of functional groups, crystallinity and morphological behavior of zinc ferrite particles was done by using Fourier transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy, respectively. Synthesized particles were activated by glutaraldehyde and enzyme was immobilized via covalent adsorption. The maximum laccase loading was achieved with 40 mg/100 mg of support and 6 hours of incubation period. Immobilized laccase displayed high immobilization yield and apparent activity i.e. 89.6% and 70% respectively. The pH and temperature optima for the free and immobilized enzymes were observed to be same, however, immobilized laccase exhibited significantly higher relative activities at broader pH and temperature ranges compared to free enzyme. Immobilization of enzyme resulted in improved thermo-stability with significantly higher half-lives (i.e., 1.25–2 folds) at different temperatures. Moreover, after ten cycles of reuse the Immobilized laccase retained about 82% of its initial activity. Overall, it is revealed that laccase immobilization on zinc ferrite nano-particles has various potential characteristics making it a promising candidate for the utilization in various chemical processes.

Graphical Abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. Rehman S, Wang P, Bhatti HN, Bilal M, Asgher M (2017) Improved catalytic properties of Penicillium notatum lipase immobilized in nanoscale silicone polymeric films. Int J Biol Macromol 97:279–286

    Article  CAS  PubMed  Google Scholar 

  2. Sun K, Li S, Si Y, Huang Q (2021) Advances in laccase-triggered anabolism for biotechnology applications. Crit Rev Biotechnol 41(7):969–993

    Article  CAS  PubMed  Google Scholar 

  3. Backes E, Kato CG, Corrêa RCG, Moreira RDFPM, Peralta RA, Barros L, Peralta RM (2021) Laccases in food processing: current status, bottlenecks and perspectives. Trends Food Sci Technol 115:445–460

    Article  CAS  Google Scholar 

  4. Hussain A, Bilal M, Rafeeq H, Jabeen Z, Afsheen N, Sher F, Iqbal HM (2022) Role of laccase in the pulp and paper industry. Nanotechnology in paper and wood engineering. Elsevier, New York, pp 35–60

    Chapter  Google Scholar 

  5. Kadam AA, Saratale GD, Ghodake GS, Saratale RG, Shahzad A, Magotra VK, Sung JS (2022) Recent advances in the development of laccase-based biosensors via nano-immobilization techniques. Chemosensors 10(2):58–85

    Article  CAS  Google Scholar 

  6. Patila M, Athanasiou PE, Kortessis L, Potsi G, Kouloumpis A, Gournis D, Stamatis H (2022) Immobilization of laccase on hybrid super-structured nanomaterials for the decolorization of phenolic dyes. Processes 10(2):233–247

    Article  CAS  Google Scholar 

  7. Mahmoodi NM, Saffar-Dastgerdi MH (2020) Clean Laccase immobilized nanobiocatalysts (graphene oxide-zeolite nanocomposites): from production to detailed biocatalytic degradation of organic pollutant. Appl Catal B 268:118443–118455

    Article  CAS  Google Scholar 

  8. Nemiwal M, Zhang TC, Kumar D (2022) Enzyme immobilized nanomaterials as electrochemical biosensors for detection of biomolecules. Enzyme Microbial Technol 110006–110017.

  9. Mohajershojaei K, Khosravi A, Mahmoodi NM (2014) Decolorization of dyes using immobilized laccase enzyme on zinc ferrite nanoparticle from single and binary systems. Fibers Polym 15(10):2139–2145

    Article  CAS  Google Scholar 

  10. Patel SK, Gupta RK, Kim SY, Kim IW, Kalia VC, Lee JK (2021) Rhus vernicifera laccase immobilization on magnetic nanoparticles to improve stability and its potential application in bisphenol a degradation. Indian J Microbiol 61(1):45–54

    Article  CAS  PubMed  Google Scholar 

  11. Verma NK, Raghav N (2021) Comparative study of covalent and hydrophobic interactions for α-amylase immobilization on cellulose derivatives. Int J Biol Macromol 174:134–143

    Article  CAS  PubMed  Google Scholar 

  12. Srinivasan P, Selvankumar T, Paray BA, Rehman MU, Kamala-Kannan S, Govarthanan M, Selvam K (2020) Chlorpyrifos degradation efficiency of Bacillus sp. laccase immobilized on iron magnetic nanoparticles. 3 Biotech 10(8):1–11

    Article  Google Scholar 

  13. Mahmoodi NM, Oveisi M, Bakhtiari M, Hayati B, Shekarchi AA, Bagheri A, Rahimi S (2019) Environmentally friendly ultrasound-assisted synthesis of magnetic zeolitic imidazolate framework-graphene oxide nanocomposites and pollutant removal from water. J Mol Liq 282:115–130

    Article  CAS  Google Scholar 

  14. Kaur M, Kaur N (2016) Ferrites: synthesis and applications for environmental remediation. In: Ferrites and ferrates: chemistry and applications in sustainable energy and environmental remediation. American Chemical Society, pp 113–136

  15. Vinosha PA, Mely LA, Jeronsia JE, Krishnan S, Das SJ (2017) Synthesis and properties of spinel ZnFe2O4 nanoparticles by facile co-precipitation route. Optik 134:99–108

    Article  CAS  Google Scholar 

  16. Kumar R, Kaur J, Jain S, Kumar A (2016) Optimization of laccase production from Aspergillus flavus by design of experiment technique: partial purification and characterization. J Genetic Eng Biotechnol 14(1):125–131

    Article  Google Scholar 

  17. Baldrian P (2006) Fungal laccases–occurrence and properties. FEMS Microbiol Rev 30(2):215–242

    Article  CAS  PubMed  Google Scholar 

  18. Min KL, Kim YH, Kim YW, Jung HS, Hah YC (2001) Characterization of a novel laccase produced by the wood-rotting fungus Phellinus ribis. Arch Biochem Biophys 392(2):279–286

    Article  CAS  PubMed  Google Scholar 

  19. Patel H, Gupte S, Gahlout M, Gupte A (2014) Purification and characterization of an extracellular laccase from solid-state culture of Pleurotus ostreatus HP-1. 3 Biotech 4(1):77–84

    Article  PubMed  Google Scholar 

  20. Patel SK, Anwar MZ, Kumar A, Otari SV, Pagolu RT, Kim SY, Lee JK (2018) Fe2O3 yolk-shell particle-based laccase biosensor for efficient detection of 2, 6-dimethoxyphenol. Biochem Eng J 132:1–8

    Article  CAS  Google Scholar 

  21. Mahmoodi NM (2013) Zinc ferrite nanoparticle as a magnetic catalyst: synthesis and dye degradation. Mater Res Bull 48(10):4255–4260

    Article  CAS  Google Scholar 

  22. Nejati K, Zabihi R (2012) Preparation and magnetic properties of nano size nickel ferrite particles using hydrothermal method. Chem Cent J 6(1):1–6

    Article  Google Scholar 

  23. Reshmi R, Sanjay G, Sugunan S (2006) Enhanced activity and stability of α-amylase immobilized on alumina. Catal Commun 7(7):460–465

    Article  CAS  Google Scholar 

  24. Sharma RP, Raut SD, Kadam AS, Mulani RM, Mane RS (2020) In-vitro antibacterial and anti-biofilm efficiencies of chitosan-encapsulated zinc ferrite nanoparticles. Appl Phys A 126(10):1–9

    Article  Google Scholar 

  25. Andjelković L, Šuljagić M, Lakić M, Jeremić D, Vulić P, Nikolić AS (2018) A study of the structural and morphological properties of Ni–ferrite, Zn–ferrite and Ni–Zn–ferrites functionalized with starch. Ceram Int 44(12):14163–14168

    Article  Google Scholar 

  26. Okoroh DO, Aisida SO, Asogwa PU (2018) Synthesis and characterization of biopolymer capped zinc ferrite nanoparticles by a thermal treatment method. IOSR J Appl Phys 10:52–56

    Google Scholar 

  27. Wen X, Du C, Wan J, Zeng G, Huang D, Yin L, Zhang J (2019) Immobilizing laccase on kaolinite and its application in treatment of malachite green effluent with the coexistence of Cd (П). Chemosphere 217:843–850

    Article  CAS  PubMed  Google Scholar 

  28. Muthuvelu KS, Rajarathinam R, Selvaraj RN, Rajendren VB (2020) A novel method for improving laccase activity by immobilization onto copper ferrite nanoparticles for lignin degradation. Int J Biol Macromol 152:1098–1107

    Article  PubMed  Google Scholar 

  29. Chen X, He B, Feng M, Zhao D, Sun J (2020) Immobilized laccase on magnetic nanoparticles for enhanced lignin model compounds degradation. Chin J Chem Eng 28(8):2152–2159

    Article  CAS  Google Scholar 

  30. Zhang D, Deng M, Cao H, Zhang S, Zhao H (2017) Laccase immobilized on magnetic nanoparticles by dopamine polymerization for 4-chlorophenol removal. Green Energy Environ 2(4):393–400

    Article  Google Scholar 

  31. Yang WY, Wen SX, Jin L, Rong L, Tetsuo M, Bo C (2006) Immobilization and characterization of laccase from Chinese Rhus vernicifera on modified chitosan. Process Biochem 41(6):1378–1382

    Article  CAS  Google Scholar 

  32. Wu E, Li Y, Huang Q, Yang Z, Wei A, Hu Q (2019) Laccase immobilization on amino-functionalized magnetic metal organic framework for phenolic compound removal. Chemosphere 233:327–335

    Article  CAS  PubMed  Google Scholar 

  33. Wang F, Guo C, Yang LR, Liu CZ (2010) Magnetic mesoporous silica nanoparticles: fabrication and their laccase immobilization performance. Biores Technol 101(23):8931–8935

    Article  CAS  Google Scholar 

  34. Tarasi R, Alipour M, Gorgannezhad L, Imanparast S, Yousefi-Ahmadipour A, Ramezani A, Khoobi M (2018) Laccase immobilization onto magnetic β-cyclodextrin-modified chitosan: improved enzyme stability and efficient performance for phenolic compounds elimination. Macromol Res 26(8):755–762

    Article  CAS  Google Scholar 

  35. Yang J, Wang Z, Lin Y, Ng TB, Ye X, Lin J (2017) Immobilized Cerrena sp. laccase: preparation, thermal inactivation, and operational stability in malachite green decolorization. Sci Rep 7(1):1–9

    Google Scholar 

  36. Bilal M, Asgher M, Shahid M, Bhatti HN (2016) Characteristic features and dye degrading capability of agar-agar gel immobilized manganese peroxidase. Int J Biol Macromol 86:728–740

    Article  CAS  PubMed  Google Scholar 

  37. Georgieva S, Godjevargova T, Portaccio M, Lepore M, Mita DG (2008) Advantages in using non-isothermal bioreactors in bioremediation of water polluted by phenol by means of immobilized laccase from Rhus vernicifera. J Mol Catal B 55(3–4):177–184

    Article  CAS  Google Scholar 

  38. Zhang C, You S, Liu Y, Wang C, Yan Q, Qi W, He Z (2020) Construction of luffa sponge-based magnetic carbon nanocarriers for laccase immobilization and its application in the removal of bisphenol A. Biores Technol 305:123085–123093

    Article  CAS  Google Scholar 

  39. Fortes CC, Daniel-da-Silva AL, Xavier AM, Tavares AP (2017) Optimization of enzyme immobilization on functionalized magnetic nanoparticles for laccase biocatalytic reactions. Chem Eng Process 117:1–8

    Article  CAS  Google Scholar 

  40. Patel SK, Otari SV, Li J, Kim DR, Kim SC, Cho BK, Lee JK (2018) Synthesis of cross-linked protein-metal hybrid nanoflowers and its application in repeated batch decolorization of synthetic dyes. J Hazard Mater 347:442–450

    Article  CAS  PubMed  Google Scholar 

  41. Ran F, Zou Y, Xu Y, Liu X, Zhang H (2019) Fe3O4@ MoS2@ PEI-facilitated enzyme tethering for efficient removal of persistent organic pollutants in water. Chem Eng J 375:121947–121955

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The analytical facilities used in the study were provided by Central Hitech Lab, Government College University, Faisalabad. The authors are thankful to the lab for providing the facilities.

Funding

The authors received support in terms of resources, instruments and equipments etc. from Government College University Faisalabad. No funding was received to assist with the preparation of this manuscript. No funding was received for conducting this study.

Author information

Authors and Affiliations

  1. Department of Chemistry, Government College University, Faisalabad, Pakistan

    Muhammad Awais, Farhat Ijaz, Mazhar Rafique & Saima Rehman

  2. Department of Biochemistry, Government College University, Faisalabad, Pakistan

    Shagufta Kamal

Authors
  1. Muhammad Awais
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shagufta Kamal
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Farhat Ijaz
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mazhar Rafique
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Saima Rehman
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by [MA], [SK], [SR], [FI] and [MR]. The first draft of the manuscript was written by [MA] and [SR] and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Saima Rehman.

Ethics declarations

Conflict of interest

The authors have no conflicts of interest to declare that are relevant to the content of this article.

Data Availability

Not Applicable.

Code Availability

Software application or custom code—Not Applicable.

Ethics Approval

The study has been conducted keeping in view the ethical considerations. No human or animal trials were included.

Consent to Participate

Not Applicable.

Consent for Publication

All the authors have consent to publish the article in Catalysis letters.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Awais, M., Kamal, S., Ijaz, F. et al. Improved Catalytic Performance of Aspergillus flavus Laccase Immobilized on the Zinc Ferrite Nanoparticles. Catal Lett 153, 1240–1249 (2023). https://doi.org/10.1007/s10562-022-04067-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10562-022-04067-3

Keywords

Search

Navigation