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Bioprocess Scale-up for Acetohydroxamic Acid Production by Hyperactive Acyltransferase of Immobilized Rhodococcus Pyridinivorans

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Abstract

In this study, Rhodococcus pyridinivorans cells containing hyperactive acyltransferase was immobilized on various macromolecules based-polymeric matrices and used to improve acetohydroxamic acid production. The calcium-alginate-based matrix retained the maximum residual activity up to 97.8% as compared to free cells (576 U/mg of dry cell weight). After immobilization, cells exhibited a significant improvement in their tolerance towards pH, temperature, and metal ions as potent enzyme inhibitors. Immobilized cells showed 25.5-fold higher thermal stability at 60 °C to control (free cells). Compared to free cells, immobilized cells exhibited a high bioconversion of acetamide and hydroxylamine-HCl to acetohydroxamic acid up to 96% molar conversion. Repeated bench-scale production at 3-L culture, immobilized cells showed 9.5-fold higher residual conversion as compared to control (100%), after five cycles of reuses. The product characterization achieved high purity (97%) of acetohydroxamic acid. This finding showed high feasibility to achieve efficient conversion that can be scaled up to the industrial level for biotechnological application.

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Acknowledgements

The authors are highly grateful to the Department of Biotechnology, Himachal Pradesh University, Shimla, India, for providing the laboratory and chemical facilities during the study. Computational Facility of Bioinformatics Centre, Himachal Pradesh University Shimla is also duly acknowledged.

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Authors and Affiliations

  1. Department of Biotechnology, Himachal Pradesh University, Shimla, 171005, India

    Neena Devi, Sanjay K. S. Patel, Nandita Thakur, Jeevan Lata & Duni Chand

  2. Department of Biotechnology, Shoolini University of Biotechnology and Management Sciences, Solan, 173229, India

    Pradeep Kumar

  3. Department of Bioinformatics, Banaras Hindu University, Mahila Mahavidyalaya, Varanasi, 221005, India

    Archana Singh

  4. Department of Reproductive Biology, All India Institute of Medical Sciences, New Delhi, 110029, India

    Deepak Pandey

  5. Department of Virology, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, India

    Vikram Thakur

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  1. Neena Devi
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Correspondence to Duni Chand.

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Devi, N., Patel, S.K.S., Kumar, P. et al. Bioprocess Scale-up for Acetohydroxamic Acid Production by Hyperactive Acyltransferase of Immobilized Rhodococcus Pyridinivorans. Catal Lett 152, 944–953 (2022). https://doi.org/10.1007/s10562-021-03696-4

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