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Bioproduction of l-2-Aminobutyric Acid by a Newly-Isolated Strain of Aspergillus tamarii ZJUT ZQ013

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Abstract

l-2-Aminobutyric acid (l-ABA), an unnatural amino acid, is a key intermediate of several important drugs. Although some methods have been developed to prepare pure chiral l-ABA, there are still many drawbacks, including low catalytic efficiency, cumbersome steps and high cost due to the addition of some expensive catalysts or coenzymes. Herein, with chemical and biological approaches together, we discovered a newly isolated Aspergillus tamarii ZJUT ZQ013 strain containing a microbial lipase which could be employed to resolve racemic methyl N-Boc-2-aminobutyrate to produce l-ABA with high enantioselectivity (e.e.s > 99.9%, E = 257). Moreover, the subsequent gram scale experiment confrimed that A. tamarii ZJUT ZQ013 could be an attractive biocatalyst for the efficient preparation of optically pure acid.

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Acknowledgements

This work was supported by National Science Foundation of China (Nos. 21272212, 21472172) and Zhejiang Natural Science Fund (Nos. LY17B060009, LY12B02018), Project of Science Technology Department of Zhejiang Province (2014C33141), and Project of Department of Science Technology Jinhua City (2013-3-003).

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  1. Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Hangzhou, 310014, China

    Zhengfang An, Xiaoxu Gu, Yue Liu, Jingyan Ge & Qing Zhu

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  1. Zhengfang An
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  2. Xiaoxu Gu
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  3. Yue Liu
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  4. Jingyan Ge
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  5. Qing Zhu
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Correspondence to Qing Zhu.

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An, Z., Gu, X., Liu, Y. et al. Bioproduction of l-2-Aminobutyric Acid by a Newly-Isolated Strain of Aspergillus tamarii ZJUT ZQ013. Catal Lett 147, 837–844 (2017). https://doi.org/10.1007/s10562-017-1999-3

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  • DOI: https://doi.org/10.1007/s10562-017-1999-3

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