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Immunomagnetic bead-based cell concentration microdevice for dilute pathogen detection

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Abstract

A cell concentration microdevice for immunomagnetic pathogen isolation from a dilute sample is presented. Cells are driven by integrated on-chip pumps through a fluidized bed of immobilized immunomagnetic beads. Off-chip polymerase chain reaction and capillary electrophoretic analysis are used to determine capture efficiencies of E. coli and to optimize the system. Beads are immobilized after each split in a bifurcated channel system to ensure a balanced distribution of beads in all the capture channels. The addition of a pumping flutter step to repeatedly drive sample through the bead bed was found to enhance capture. Capture efficiencies of 70% and a limit of detection of 2 cfu/μL were achieved; specific capture of E. coli at a concentration of 100 cfu/μL in a 100-fold background of S. aureus is shown. This capture/concentration system is an important step in overcoming the macro-to-micro interface challenge in the development of microdevices for pathogen detection.

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Acknowledgements

We appreciate the assistance of Kelly Flook, Nick Toriello, George Sensabaugh and James Yang throughout this project. All microfabrication was performed at the UC Berkeley Microfabrication Laboratory. This research was supported by the NIH (#U01AI056472).

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Authors and Affiliations

  1. UCSF/UC Berkeley Joint Graduate Group in Bioengineering, University of California, Berkeley, CA, 94720, USA

    Nathaniel Beyor, Peng Liu & Richard A. Mathies

  2. Department of Chemistry, University of California, Berkeley, CA, 94720, USA

    Tae Seok Seo & Richard A. Mathies

  3. Department of Chemical & Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Daejeon, 305-701, Korea

    Tae Seok Seo

Authors
  1. Nathaniel Beyor
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  2. Tae Seok Seo
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  3. Peng Liu
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  4. Richard A. Mathies
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Corresponding author

Correspondence to Richard A. Mathies.

Additional information

Nathaniel Beyor and Tae Seok Seo contributed equally to this work

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Beyor, N., Seo, T.S., Liu, P. et al. Immunomagnetic bead-based cell concentration microdevice for dilute pathogen detection. Biomed Microdevices 10, 909–917 (2008). https://doi.org/10.1007/s10544-008-9206-3

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  • DOI: https://doi.org/10.1007/s10544-008-9206-3

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