Abstract
The anther culture technique, although it has been widely used in wheat breeding programs, is associated with a high albinism rate that limits its application. The mechanism of albinism has not been studied explicitly at the molecular level. In this study, differently expressed genes (DEGs) and proteins (DEPs) of albino and green seedlings from a wheat anther culture were analyzed and compared using transcriptomic sequencing (RNA-Seq), two-dimensional electrophoresis, and mass spectrometry. A total of 1 892 DEGs (1 115 down-regulated and 777 up-regulated) and 43 DEPs (15 down-regulated and 28 up-regulated) that are primarily involved in photosynthetic pathways and porphyrin and chlorophyll metabolism was identified when the albino group library was used as the control. Most DEGs participated in constructing components of thylakoids and the chloroplast envelope as determined via gene ontology analysis. The expression patterns of 12 selected genes were evaluated using real-time quantitative PCR, and the results were in agreement with the RNA-Seq analysis. Our results may assist the development of new methods to decrease the albino seedling rates in wheat anther cultures.
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Abbreviations
- 2-DE:
-
two-dimensional electrophoresis
- CI:
-
confidence interval
- DEGs:
-
differently expressed genes
- DEPs:
-
differently expressed proteins
- GO:
-
gene ontology
- KEGG:
-
Kyoto encyclopedia of genes and genomes
- MS:
-
mass spectrometry
- PS:
-
photosystem
- RPKM:
-
reads per kilo base per million reads
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Acknowledgments: This research was financially supported by grants in part from the National Natural Science Foundation of China (31401380 and 31371621) and from the Ministry of Agriculture in China (2014ZX08010-004). The first two authors contributed equally to this work.
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Zhao, P., Wang, K., Zhang, W. et al. Comprehensive analysis of differently expressed genes and proteins in albino and green plantlets from a wheat anther culture. Biol Plant 61, 255–265 (2017). https://doi.org/10.1007/s10535-016-0662-y
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DOI: https://doi.org/10.1007/s10535-016-0662-y