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Expression of DORMANCY-ASSOCIATED MADS-BOX (DAM)-like genes in apple

  • Original Papers
  • Published:
Biologia Plantarum

Abstract

Apple (Malus × domestica Borkh.) is a perennial woody plant that undergoes a period of dormancy (in cv. Jonathan between late September and mid-December) to survive freezing temperatures of winter. DORMANCY-ASSOCIATED MADS-BOX (DAM) genes play important roles in the regulation of growth cessation and terminal bud formation in peach. To understand the role of DAM orthologs in apple, we isolated and characterized four DAM-like genes (designated as MdDAMa, MdDAMb, MdDAMc, and MdDAMd) and monitored their expression in apical buds throughout the season by real-time quantitative polymerase chain reaction analyses. The transcription of MdDAMa peaked in October and that of MdDAMc was elevated from August to October, whereas MdDAMb and MdDAMd were practically undetectable. The tandemly arranged genes MdDAMa/MdDAMb and MdDAMc/MdDAMd were localized to chromosomes 16 and 8, respectively. Based on these observations, we infer that MdDAMa and MdDAMc acted in a dominant fashion on each locus and were correlated with the period of endodormancy.

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Abbreviations

CBF:

C-REPEAT BINDING FACTOR

CENL1:

CENTRORADIALIS-LIKE1

FLC:

FLOWERING LOCUS C

GDR:

genome database for Rosaceae

FT:

FLOWERING LOCUS T

Md:

Malus × domestica

PEBP:

phosphatidyl-ethanolamine binding protein

PlnTFDB:

plant transcription factor database

QTL:

quantitative trait locus

RT-qPCR:

real time quantitative polymerase chain reaction

SVP:

SHOOT VEGETATIVE PHASE

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Author notes

  1. N. Mimida

    Present address: Gene Research Center, University of Tsukuba, Tsukuba, Ibaraki, 305-8752, Japan

Authors and Affiliations

  1. Faculty of Agriculture, Iwate University, Morioka, Iwate, 020-8550, Japan

    N. Mimida, A. Suzuki & S. Komori

  2. Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305-8572, Japan

    T. Saito & T. Moriguchi

  3. Department of Plant, Cell and Environment, NARO Institute of Fruit Tree Science, Tsukuba, Ibaraki, 305-8605, Japan

    T. Saito & T. Moriguchi

  4. Apple Research Division, NARO Institute of Fruit Tree Science, Morioka, Iwate, 020-0123, Japan

    M. Wada

Authors
  1. N. Mimida
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  2. T. Saito
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  3. T. Moriguchi
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  4. A. Suzuki
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  5. S. Komori
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  6. M. Wada
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Corresponding authors

Correspondence to N. Mimida or M. Wada.

Additional information

Acknowledgement: This work was supported by a grant-in-aid (No. 15208004) from the Ministry of Education, Science and Culture of Japan.

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Mimida, N., Saito, T., Moriguchi, T. et al. Expression of DORMANCY-ASSOCIATED MADS-BOX (DAM)-like genes in apple. Biol Plant 59, 237–244 (2015). https://doi.org/10.1007/s10535-015-0503-4

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  • DOI: https://doi.org/10.1007/s10535-015-0503-4

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