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Extracellular ATP alleviates the salicylic acid-induced inhibition of cell viability and respiration through a Ca2+-dependent mechanism

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  • Published:
Biologia Plantarum

Abstract

Extracellular ATP (eATP) has been considered as signalling compound to mediate several physiological processes. Here we show that eATP played a role in alleviating the salicylic acid (SA)-induced inhibition of cell viability and respiration in tobacco (Nicotiana tabacum L. cv. Bright Yellow-2) suspension cells. Exogenous SA at higher concentrations (0.05, 0.1, 0.3, 0.5, or 0.7 mM) caused a significant reduction in respiratory O2 uptake or cell viability. The addition of exogenous ATP alleviated the SA-induced reductions in cell viability and respiration, but the effect was dependent on the concentrations of either exogenous SA or ATP. Further study shows that the alleviative effects of exogenous ATP were abolished by the addition of GdCl3 (an inhibitor of Ca2+ channels) or EGTA (a Ca2+ chelator). These results suggest that this role of extracellular ATP could be related to a Ca2+ signalling pathway.

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Abbreviations

eATP:

extracellular ATP

SA:

salicylic acid

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Authors and Affiliations

  1. College of Life Science, Northwest Normal University, Lanzhou, 730070, Gansu, P.R. China

    H. Q. Feng, D. D. Guan, J. Y. Bai, L. Y. Jia, Y. Fang & K. Sun

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  1. H. Q. Feng
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  2. D. D. Guan
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  3. J. Y. Bai
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  4. L. Y. Jia
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  5. Y. Fang
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  6. K. Sun
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Corresponding authors

Correspondence to H. Q. Feng or K. Sun.

Additional information

Acknowledgements: We thank Prof. Jiang Liwen (The Chinese University of Hong Kong) for the supply of the tobacco cell culture. This work was supported by the National Natural Science Foundation of China (Nos. 31260059 and 30900105), the Key Project of Chinese Ministry of Education (No. 211190), the Fundamental Research Funds for the Gansu Universities of Gansu Provincial Department of Finance, and NWNU-kjcxgc-03-77 and 49.

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Feng, H.Q., Guan, D.D., Bai, J.Y. et al. Extracellular ATP alleviates the salicylic acid-induced inhibition of cell viability and respiration through a Ca2+-dependent mechanism. Biol Plant 59, 193–197 (2015). https://doi.org/10.1007/s10535-014-0464-z

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  • DOI: https://doi.org/10.1007/s10535-014-0464-z

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