Abstract
Increased aldose reductase (ALR) activities were detected in the leaf tissues of tomato plants grown for 3 weeks in culture medium containing 10−7 or 10−4 M salicylic acid (SA), and in the roots after the 10−4 M SA pretreatment. The ALR activity changed in parallel with the sorbitol content in the leaves of the SA-treated plants. Salt stress elicited by 100 mM NaCl enhanced the accumulation of sorbitol in the leaves of control plants and as compared with the untreated control the sorbitol content in the SA-pretreated leaves remained elevated under salt stress. DEAE cellulose anionexchange column purification of the protein precipitated with 80 % (NH4)2SO4 revealed two enzyme fractions with ALR activity in both the leaf and the root tissues. The fraction of the leaf extract that was not bound to the column reacted with glucose and glucose-6-P as substrates, whereas glucose was not a substrate for the bound fraction or for root isoenzymes. The root enzyme was less sensitive to salt treatment: 50 mM NaCl caused 30 % inhibition in the leaf extract, whereas the enzyme activity of the root extract was not affected. It is suggested that increased ALR activity and sorbitol synthesis in the leaves of SA-treated tomato plants may result in an improved salt stress tolerance.
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Abbreviations
- ABA:
-
abscisic acid
- ALR:
-
aldose reductase
- 4-HNE:
-
4-hydroxynon-2-enal
- RAs:
-
reactive aldehydes
- ROS:
-
reactive oxygen species
- SA:
-
salicylic acid
- S6PHD:
-
sorbitol-6-phosphate dehydrogenase
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Acknowledgements
We thank Kispálné Szabó Ibolya for her excellent technical assistance. This work was supported by grants from the Hungarian National Scientific Research Foundation (OTKA TO38392 and OTKA K76854).
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Tari, I., Kiss, G., Deér, A.K. et al. Salicylic acid increased aldose reductase activity and sorbitol accumulation in tomato plants under salt stress. Biol Plant 54, 677–683 (2010). https://doi.org/10.1007/s10535-010-0120-1
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DOI: https://doi.org/10.1007/s10535-010-0120-1