Skip to main content
SpringerLink
Log in

Effect of aluminum on the in vitro activity of acid phosphatases of four potato clones grown in three growth systems

  • Brief Communication
  • Published:
Biologia Plantarum

Abstract

The aim of this study was to assess the effect of aluminum on the in vitro activity of acid phosphatases (APases) of four potato clones, Macaca and Dakota Rose (Al-sensitive), and SMIC148-A and Solanum microdontum (Al-tolerant), grown in vitro, in hydroponics or in a greenhouse. The enzyme was assayed in vitro in the presence of 0, 1.85, 3.70, 5.55 and 7.40 mM Al. In plantlets grown in vitro, root APases were inhibited by Al in all clones, while shoot APases were inhibited by Al in S. microdontum and Dakota Rose and increased in Macaca at all Al concentrations. In plantlets grown in hydroponics, root APases increased in Macaca at 1.85 mM Al, whereas decreased at all Al levels in S. microdontum. In greenhouse plantlets, root APases decreased at 7.40 mM Al in S. microdontum and SMIC148-A, and at 3.70, 5.55 and 7.40 mM Al in Dakota Rose. Shoot APases decreased in Macaca and SMIC148-A. Conversely, in Dakota Rose, APases increased at 1.85 and 3.70 mM Al. These results show that the effect of Al toxicity on in vitro APase activity depends not only on Al availability but also on the plant organ, genetic background, and the growth conditions. Therefore, it suggests that acid phosphatases activity assessed in vitro might not be a good parameter to validate the screening for adaptation of potato clones to Al toxicity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Abbreviations

APase:

phosphatase

EDTA:

ethylenediaminetetraacetic acid

Pi:

inorganic phosphate

TCA:

trichloroacetic acid

References

  • Baroja, M.E., Aguirreolea, J., Sánchez-Díaz, M.: CO2 exchange of in vitro and acclimatized potato plantlets. — In: Carre, F., Chagvardieff, P. (ed.): Ecophysiology and Photosynthetic in vitro Cultures. Pp. 187–188. Centre d’Études de Cadarache, Saint-Paul-lez-Durance 1995.

    Google Scholar 

  • Bozzo, G.G., Raghothama, K.G., Plaxton, W.C.: Purification and characterization of two secreted purple acid phosphatase isozymes from phosphate-starved tomato (Lycopersicon esculentum) cell cultures. — Eur. J. Biochem. 269: 6278–6286, 2002.

    Article  CAS  PubMed  Google Scholar 

  • Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantity of protein utilizing the principle of protein-dye binding. — Anal. Biochem. 72: 248–254, 1976.

    Article  CAS  PubMed  Google Scholar 

  • Delhaize, E., Ryan, P.R.: Aluminum toxicity and tolerance in plants. — Plant Physiol. 107: 315–321, 1995.

    CAS  PubMed  Google Scholar 

  • Del Pozo, J.C., Allona, I., Rubio, V., Leyva, A., De la Peña, A., Aragoncillo, C., Paz-Ares, J.: A type 5 acid phosphatase gene from Arabidopsis thaliana is induced by phosphate starvation and by some other types of phosphate mobilizing/oxidative stress conditions. — Plant J. 19: 579–589, 1999.

    Article  PubMed  Google Scholar 

  • Dong, B., Sang, W.L., Jiang, X., Zhou, J.M., Kong, F.X., Hu, W., Wang, L.S.: Effects of aluminum on physiological metabolism and antioxidant system of wheat (Triticum aestivum L.). — Chemosphere 47: 87–92, 2002.

    Article  CAS  PubMed  Google Scholar 

  • Duff, S.M.G., Sarath, G., Plaxton, W.C.: The role of acid phosphatase in plant phosphorus metabolism. — Physiol. Plant. 90: 791–800, 1994.

    Article  CAS  Google Scholar 

  • Eticha, D., Thé, C., Welcker, C., Narro, L., Staß, A., Horst, W.J.: Aluminium-induced callose formation in root apices: inheritance and selection trait for adaptation of tropical maize to acid soils. — Field Crops Res. 93: 252–263, 2005.

    Article  Google Scholar 

  • Gabbrielli, R., Grossi, L., Vergnano, O.: The effects of nickel, calcium and magnesium on the acid phosphatase activity of two Alyssum species. — New Phytol. 111: 631–636, 1989.

    Article  CAS  Google Scholar 

  • Goodwin, S.B., Sutter, T.R.: Microarray analysis of Arabidopsis genome response to aluminum stress. — Biol. Plant. 53: 85–99, 2009.

    Article  CAS  Google Scholar 

  • Hairiah, K., Noordwijk, J., Setijono, S.: Tolerance and avoidance of Al toxicity by Mucuna pruriens var. utilis at different levels of P supply. — Plant Soil 171: 77–81, 1995.

    Article  CAS  Google Scholar 

  • Hazarika, B.N.: Acclimatization of tissue-cultured plants. — Curr. Sci. 85: 1704–1712, 2003.

    CAS  Google Scholar 

  • Horst, W.J., Schmohl, N., Kollmeier, M., Balułka, F., Sivaguru, M.: Does aluminium affect root growth of maize through interaction with the cell wall — plasma membrane — cytoskeleton continuum? — Plant Soil 215: 163–174, 1999.

    Article  CAS  Google Scholar 

  • Kochian, L.V., Hoekenga, O.A., Piñeros, M.A.: How do crop plants tolerate acid soils? Mechanisms of aluminum tolerance and phosphorous efficiency. — Annu. Rev. Plant Biol. 55: 459–493, 2004.

    Article  CAS  PubMed  Google Scholar 

  • Liu, Q., Yang, J.L., He, L.S., Li, Y.Y., Zheng, S.J.: Effect of aluminum on cell wall, plasma membrane, antioxidants and root elongation in triticale. — Biol. Plant. 52: 87–92, 2008.

    Article  CAS  Google Scholar 

  • Luhová, L., Lebeda, A., Kutrová, E., Hederervá, D., Peç, P.: Peroxidase, catalase, amine oxidase and acid phosphatase activities in Pisum sativum during infection with Fusarium oxysporum and F. solani. — Biol. Plant. 50: 675–682, 2006.

    Article  Google Scholar 

  • Marschner, H. (ed.): Mineral Nutrition of Higher Plants. — Academic Press, London 1995.

    Google Scholar 

  • Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. — Physiol. Plant. 15: 473–497, 1962.

    Article  CAS  Google Scholar 

  • Pereira, L.B., Tabaldi, L.A., Gonçalves, J.F., Jucoski, G.O., Pauletto, M.M., Weis, S.N., Nicoloso, F.T., Borher, D., Rocha, J.B.T., Schetinger, M.R.C.: Effect of aluminum on δ-aminolevulinic acid dehydratase (ALA-D) and the development of cucumber (Cucumis sativus). — Environ. exp. Bot. 57: 106–115, 2006.

    Article  CAS  Google Scholar 

  • Pospíšilová, J., Tichá, I., Kadleček, P., Haizel, D., Plzáková, Š.: Acclimatization of micropropagated plants to ex vitro conditions. — Biol. Plant. 42: 481–497, 1999.

    Article  Google Scholar 

  • Rai, L.C., Husaini, Y., Mallick, N.: pH-altered interaction of aluminum and fluoride on nutrient uptake, photosynthesis and other variables of Chlorella vulgaris. — Aquat. Toxicol. 42: 67–84, 1998.

    Article  CAS  Google Scholar 

  • Shamsi, I.H., Wei, K., Zhang, G.P., Jilani, G.H., Hassan, M.J.: Interactive effects of cadmium and aluminum on growth and antioxidative enzymes in soybean. — Biol. Plant. 52: 165–169, 2008.

    Article  CAS  Google Scholar 

  • Tabaldi, L.A., Cargnelutti, D., Gonçalves, J.F., Pereira, L.B., Castro, G.Y., Maldaner, J., Rauber, R., Rossato, L.V., Bisognin, D.A., Schetinger, M.R.C., Nicoloso, F.T.: Oxidative stress is an early symptom triggered by aluminum in Al-sensitive potato plantlets. — Chemosphere 76: 1402–1409, 2009b.

    Article  CAS  PubMed  Google Scholar 

  • Tabaldi, L.A., Castro, G.Y, Cargnelutti, D., Skrebsky, E.C., Gonçalves, J.F., Rauber, R., Rossato, L.V., Schetinger, M.R.C., Bisognin, D.A., Nicoloso, F.T.: Micronutrient concentration in potato clones with distinct physiological sensitivity to Al stress. — Cienc. Rural 39: 379–385, 2009a.

    Article  CAS  Google Scholar 

  • Tabaldi, L.A., Nicoloso, F.T., Castro, G.Y, Cargnelutti, D., Gonąlves, J.F., Rauber, R., Skrebsky, E.C., Schetinger, M.R.C., Morsch, V.M., Bisognin, D.A.: Physiological and oxidative stress responses of four potato clones to aluminum in nutrient solution. — Braz. J. Plant Physiol. 19: 211–222, 2007b.

    Article  CAS  Google Scholar 

  • Tabaldi, L.A., Ruppenthal, R., Cargnelutti, D., Morsch, V.M., Pereira, L.B., Schetinger, M.R.C.: Effects of metal elements on acid phosphatase activity in cucumber (Cucumis sativus L.) seedlings. — Environ. exp. Bot. 59: 43–48, 2007a.

    Article  CAS  Google Scholar 

  • Tejera Garcia, N.A., Oliveira, M., Iribarne, C., Lluch, C.: Partial purification and characterization of a non-specific acid phosphatase in leaves and root nodules of Phaseolus vulgaris. — Plant Physiol. Biochem. 42: 585–591, 2004.

    Article  CAS  PubMed  Google Scholar 

  • Yoneyama, T., Taira, M., Suzuki, T., Nakamura, M., Niwa, K., Watanabe, T., Ohyama, T.: Expression and characterization of a recombinant unique acid phosphatase from kidney bean hypocotyl exhibiting chloroperoxidase activity in the yeast Pichia pastoris. — Prot. Expression Purification 53: 31–39, 2007.

    Article  CAS  Google Scholar 

  • Yu, M., Shen, R., Xiao, H., Xu, M., Wang, H., Wang, H, Zeng, Q., Bian, J.: Boron alleviates aluminum toxicity in pea (Pisum sativum). — Plant Soil 314: 87–98, 2009.

    Article  CAS  Google Scholar 

  • Zheng, S.J., Yang, J.L.: Target sites of aluminum phytotoxicity. — Biol. Plant. 49: 321–331, 2005.

    Article  CAS  Google Scholar 

  • Zimmermann, P., Regierer, B., Kossmann, J., Frossard, E., Amrhein, N., Bucher, M.: Differential expression of three purple acid phosphatases from potato. — Plant Biol. 6: 519–528, 2004.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

The authors thank the Coordenação e Aperfeiçoamento de Pessoal de Nível Superior, Conselho Nacional de Desenvolvimento Científico e Tecnológico and Fundação de Amparo à Pesquisa de Estado do Rio Grande do Sul for the research fellowship.

Author information

Authors and Affiliations

  1. Departamento de Biologia, Centro de Ciências Naturais e Exatas (CCNE), Universidade Federal de Santa Maria (UFSM), Santa Maria, 97105-900, RS, Brasil

    L. A. Tabaldi, G. Y. Castro, J. F. Gonçalves, R. Rauber & F. T. Nicoloso

  2. Departamento de Química, CCNE, UFSM, Santa Maria, 97105-900, RS, Brasil

    D. Cargnelutti & M. R. C. Schetinger

  3. Departamento de Fitotecnia, Centro de Ciências Rurais, UFSM, Santa Maria, 97105-900, RS, Brasil

    D. A. Bisognin

Authors
  1. L. A. Tabaldi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. Cargnelutti
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. Y. Castro
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. F. Gonçalves
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. R. Rauber
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. D. A. Bisognin
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. M. R. C. Schetinger
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. F. T. Nicoloso
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to F. T. Nicoloso.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tabaldi, L.A., Cargnelutti, D., Castro, G.Y. et al. Effect of aluminum on the in vitro activity of acid phosphatases of four potato clones grown in three growth systems. Biol Plant 55, 178–182 (2011). https://doi.org/10.1007/s10535-011-0026-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10535-011-0026-6

Additional key words

Search

Navigation