Abstract
Two triticale cultivars ZC 237 (Al-resistant) and ZC 1890 (Al-sensitive) were used to investigate the effects of 30 to 100 µM Al on antioxidative enzyme activity, lipid peroxidation and cell wall composition. In ZC 1890, the root elongation was significantly inhibited after 1-h exposure to 50 µM Al, the changes in hemicellulose fraction were clearly detected after 2-h Al exposure, while the peroxidase (POD) and superoxide dismutase (SOD) activities significantly increased after 6-h exposure, and the malondialdehyde (MDA) content after 12-h exposure. The similar patterns were also found in ZC 237. Treatment of ZC 1890 with 1 mM citrate for 30 min after 3-h exposure to Al resulted in significant decrease of Al bound to cell-wall and recovery of root elongation. These results suggested that Al affected cell wall before the damage of plasma membrane, but this was not the primary cause of root elongation inhibition.
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Abbreviations
- f.m.:
-
fresh mass
- MDA:
-
malondialdehyde
- NBT:
-
nitroblue tetrazolium chloride
- POD:
-
peroxidase
- RRE:
-
relative root elongation
- SOD:
-
superoxide dismutase
- TBA:
-
thiobarbituric acid
References
Blamey, F.P.C., Nishizawa, N.K., Yoshimura, E.: Timing, magnitude, and location of initial soluble aluminum injuries to mungbean roots.-Soil Sci. Plant Nutr. 50: 67–76, 2004.
Boscolo, P.R.S., Menossi, M., Jorge, R.A.: Aluminum-induced oxidative stress in maize.-Phytochemistry 62: 181–189, 2003.
Cakmak, I., Horst, W.J.: Effect of aluminium on lipid peroxidation, superoxide dismutase, catalase, and peroxidase activities in root tips of soybean (Glycine max).-Physiol. Plant. 93: 463–468, 1991.
Chang, Y.C., Yamamoto, Y., Matsumoto, H.: Accumulation of aluminum in the cell wall pectin in cultured tobacco (Nicotiana tabacum L.) cells treated with a combination of aluminum and iron.-Plant Cell Environ. 22: 1009–1017, 1999.
Clarkson, D.T.: Interactions between aluminum and phosphorus on root surfaces and cell wall material.-Plant Soil 27: 347–356, 1967.
Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., Smith, F.: Colorimetric method for determination of sugars and related substances.-Anal. Chem. 28: 350–356, 1956.
Ezaki, B., Gardner, R.C., Ezaki, Y., Matsumoto, H.: Expression of aluminum-induced genes in transgenic Arabidopsis plants can ameliorate aluminum stress and/or oxidative stress.-Plant Physiol. 122: 657–665, 2000.
Horst, W.J.: The role of the apoplast in aluminium toxicity and distance of higher plants: A review.-Z. Pflanzenernähr. Bodenk. 158: 419–428, 1995.
Kochian, L.V.: Cellular mechanisms of aluminum toxicity and resistance in plants.-Annu. Rev. Plant Physiol. Plant mol. Biol. 46: 237–260, 1995.
Kuo, M.C., Kao C.H.: Aluminum effects on lipid peroxidation and antioxidative enzyme activities in rice leaves.-Biol. Plant. 46: 149–152, 2003.
Le Van, H., Kuraishi, S., Sakurai, N.: Aluminum-induced rapid root inhibition and changes in cell-wall components of squash seedlings.-Plant Physiol. 106: 971–976, 1994.
Llugany, M., Poschenrieder, C., Barceló, J.: Monitoring the aluminium-induced inhibition of root elongation in four maize cultivars differing in tolerance to aluminium and proton toxicity.-Physiol. Plant. 93: 265–271, 1995.
Ma, J.F., Shen, R.F., Nagao, S., Tanimoto, E.: Aluminum targets elongating cells by reducing cell wall extensibility in wheat roots.-Plant Cell Physiol. 45: 583–589, 2004.
Ma, J.F., Yamamoto, R., Nevins, D.J., Matsumoto, H., Brown, P.H.: Al binding in the epidermis cell wall inhibits cell elongation of okra hypocotyl.-Plant Cell Physiol. 40: 549–556, 1999.
Matsumoto, H.: Cell biology of aluminium toxicity and tolerance in higher plants.-Int. Rev. Cytol. 200: 1–46, 2000.
Nishitani, K., Masuda, Y.: Growth and cell wall changes in azuki bean epicotyls. I. Changes in wall polysaccharides during intact growth.-Plant Cell Physiol. 20: 63–74, 1979.
Oteiza, P.L.: A mechanism for the stimulatory effect of aluminum on iron-induced lipid peroxidation.-Arch. Biochem. Biophys. 308: 374–379, 1994.
Richards, K.D., Schott, E.J., Sharma, Y.K., Davis, K.R., Gardner, R.C.: Aluminum induces oxidative stress genes in Arabidopsis thaliana.-Plant Physiol. 116: 409–418, 1998.
Ryan, P.R., Shaff, J.E., Kochian, L.V.: Aluminum toxicity in roots: correlation among ionic currents, ion fluxes and root elongation in aluminum-sensitive and aluminum-tolerant wheat cultivars.-Plant Physiol. 99: 1193–1200, 1992.
Šimonovičová, M., Tamás, L., Huttová, J., Mistrík I.: Effect of aluminium on oxidative stress related enzymes activities in barley roots.-Biol. Plant. 48: 261–266, 2004.
Tabuchi, A., Matsumoto, H.: Changes in cell-wall properties of wheat (Triticum aestivum L.) roots during aluminum-induced growth inhibition.-Physiol. Plant. 112: 353–358, 2001.
Tamás, L., Huttová, J., Mistrík, I.: Inhibition of Al-induced root elongation and enhancement of Al-induced peroxidase activity in Al-sensitive and Al-resistant barley cultivars are positively correlated.-Plant Soil 250: 193–200, 2003.
Taylor, G.J., McDonald-Stephens, J.L., Hunter, D.B., Bertsch, P.M., Elmore, D., Rengel, Z., Reid, R.J.: Direct measurement of aluminum uptake and distribution in single cells of Chara corallina.-Plant Physiol. 123: 987–996, 2000.
Wallace, S.U., Anderson, I.C.: Aluminum toxicity and DNA synthesis in wheat roots.-Agron. J. 76: 5–8, 1984.
Yamamoto, Y., Kobayashi, Y., Devi, S.R., Rikiishi, S., Matsumoto, H.: Aluminum toxicity is associated with mitochondrial dysfunction and the production of reactive oxygen species in plant cells.-Plant Physiol. 128: 63–72, 2002.
Yamamoto, Y., Kobayashi, Y., Devi, S.R., Rikiishi, S., Matsumoto, H.: Oxidative stress triggered by aluminum in plant roots.-Plant Soil 255: 239–243, 2003.
Yamamoto, Y., Kobayashi, Y., Matsumoto, H.: Lipid peroxidation is an early symptom triggered by aluminum, but not the primary cause of elongation inhibition in pea roots.-Plant Physiol. 125: 199–208, 2001.
Yang, J.L., Zheng, S.J., He, Y.F., Tang, C.X., Zhou, G.D.: Genotypic differences among plant species in response to aluminum stress.-J. Plant Nutr. 28: 949–961, 2005.
Zheng, S.J., Yang, J.L.: Target sites of aluminum phytoxicity.-Biol. Plant. 49: 321–331, 2005.
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Liu, Q., Yang, J.L., He, L.S. et al. Effect of aluminum on cell wall, plasma membrane, antioxidants and root elongation in triticale. Biol Plant 52, 87–92 (2008). https://doi.org/10.1007/s10535-008-0014-7
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DOI: https://doi.org/10.1007/s10535-008-0014-7