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Progesterone moderates damage in Arabidopsis thaliana caused by infection with Pseudomonas syringae or P. fluorescens

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Biologia Plantarum

Abstract

Brassinosteroids are known to protect plants against various abiotic and biotic stresses, however, very limited information is available about the role of progesterone. Therefore the effects of Pseudomonas syringae pv. syringae (P.s.) wild type strain 61, its hrcC mutant, and the saprophytic P. fluorescens (P.f.) strain 55 were investigated in wild type Arabidopsis thaliana cv. Columbia and its rbohF knock-out mutant, with and without progesterone pre-treatment. The reactions of wild type and rbohF mutant Arabidopsis to bacterial inoculations were similar, although 2 h after injection of P.s. a larger increase of electrolyte leakage was measured in wild type than in rbohF knockout mutant leaves. The hrcC mutant caused weak necrotic symptoms and increased leakage in both types of Arabidopsis, although to a much lesser extent than P.s. The P.f. did not induce any visible symptom, but slightly increased the electrolyte leakage in both types of Arabidopsis. Inoculation by all Pseudomonas bacteria led to significant alterations in photosystem 2 efficiency as compared to control plants. Pre-treatment of leaves with progesterone diminished the necrotic symptoms, the electrolyte leakage and improve the efficiency of photosystem 2 caused by Pseudomonas bacteria.

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Abbreviations

BRs:

brassinosteroids

Fv/Fm :

variable to maximum fluorescence ratio

hpi:

hours post inoculation

P.f. :

Pseudomonas fluorescens

P.s. :

Pseudomonas syringae pv. syringae

PS:

photosystem

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Authors and Affiliations

  1. The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, 21 Niezapominajek, PL-30239, Kraków, Poland

    A. Janeczko, A. Skoczowski & F. Dubert

  2. Plant Protection Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Herman Otto 15, H-1022, Budapest, Hungary

    I. Tóbiás, G. Gullner & B. Barna

Authors
  1. A. Janeczko
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  2. I. Tóbiás
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  3. A. Skoczowski
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  4. F. Dubert
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  5. G. Gullner
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  6. B. Barna
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Corresponding author

Correspondence to B. Barna.

Additional information

Acknowledgements: This research was supported by the joint research project No. 25 between the Polish and Hungarian Academy of Sciences and by the Hungarian National Research Fund OTKA K83615.

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Janeczko, A., Tóbiás, I., Skoczowski, A. et al. Progesterone moderates damage in Arabidopsis thaliana caused by infection with Pseudomonas syringae or P. fluorescens . Biol Plant 57, 169–173 (2013). https://doi.org/10.1007/s10535-012-0142-y

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  • DOI: https://doi.org/10.1007/s10535-012-0142-y

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