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Early gene expression in the walnut tree occurring during stimulation of leaf hydraulic conductance by irradiance

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Biologia Plantarum

Abstract

Leaf hydraulic conductance (Kleaf) plays a significant part in plant-water regulation. In walnut leaves, Kleaf is stimulated by irradiance and closely relates to the accumulation of JrPIP2s aquaporin transcripts, but it is independent of stomatal aperture. To provide an insight into the early molecular events occurred during light-induced Kleaf, a large-scale transcriptomic analysis consisting of the cDNA-amplified fragment length polymorphism (AFLP) was carried out on walnut leaves maintained under irradiance or in darkness. Of the total 12 000 transcript-derived fragments (TDFs) obtained using cDNA-AFLP with 128 primer pairs, 187 TDFs were selected after sequencing, and only 93 (49 %) that had been ascribed known functions through BLAST searching of the GenBank databases. Most of these TDFs correspond to genes whose protein products are involved in cellular regulation (57.9 %) and global metabolism (39.8 %). To validate cDNA-AFLP expression patterns, 30 TDFs were further analyzed using real-time quantitative polymerase chain reaction. Moreover, exposure of leaves to irradiance was accompanied by the modification of the Ca2+-signaling pathway, ubiquitin-proteasome pathway, vesicle trafficking process and expression of multiple transcription factors.

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Abbreviations

AFLP:

amplified fragment length polymorphism

HPFM:

high pressure flow meter

Kleaf :

leaf hydraulic conductance

Kroot :

root hydraulic conductance

PIP:

plasma membrane intrinsic protein

PCR:

polymerase chain reaction

qRT-PCR:

quantitative real-time PCR

Rleaf :

leaf hydraulic resistance

TDF:

transcript-derived fragment

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Authors and Affiliations

  1. Clermont-Université, UMR 547 PIAF, 24 avenue des Landais, F-63177, Aubière Cedex, France

    K. Ben Bâaziz, D. Lopez, H. Cochard, J. -S. Venisse & S. Sakr

  2. UMR 547 PIAF, INRA, F-63000, Clermont-Ferrand, France

    K. Ben Bâaziz, D. Lopez, H. Cochard, J. -S. Venisse & S. Sakr

  3. Laboratoire de Biologie végétale, Faculté des Sciences de Tunis, Campus Universitaire, 1060, Tunis, Tunisia

    K. Ben Bâaziz & S. Bouzid

  4. Agrocampus Ouest, Centre d’Angers, UMR SAGAH, IFR QUASAV 149, F-49045, Angers Cedex, France

    S. Sakr

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  1. K. Ben Bâaziz
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  2. D. Lopez
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  3. S. Bouzid
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  4. H. Cochard
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  5. J. -S. Venisse
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Correspondence to S. Sakr.

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Ben Bâaziz, K., Lopez, D., Bouzid, S. et al. Early gene expression in the walnut tree occurring during stimulation of leaf hydraulic conductance by irradiance. Biol Plant 56, 657–666 (2012). https://doi.org/10.1007/s10535-012-0120-4

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  • DOI: https://doi.org/10.1007/s10535-012-0120-4

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