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Identification of genes involved in a water stress response in timothy and mapping of orthologous loci in perennial ryegrass

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Biologia Plantarum

Abstract

In order to characterize the response of selected grasses to water stress, relative water content (RWC) in leaves and quantum efficiency of photosystem 2 (Fv/Fm) were measured in Phleum pratense L., P. bertolonii DC. and P. phleoides H. Karst. during 6 d of water stress. The results indicated differential responses to water stress among the three Phleum species with higher water deficit sensitivity of P. pratense and P. bertolonii than that of P. phleoides. The cDNA-amplified fragment length polymorphism (cDNA-AFLP) technique was applied to identify differentially expressed genes responding to water stress in P. pratense. Cloned and sequenced differentially expressed fragments (DEFs) were used for primer design in order to identify orthologous genes in Lolium perenne L. Twelve genes orthologous to P. pratense DEFs were mapped in the L. perenne mapping population VrnA based on a high resolution melting curve analysis (HRM). This study provides genomic information about 29 differentially expressed genes after water stress in P. pratense and reports on the identification and mapping of twelve orthologs in L. perenne.

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Abbreviations

cDNA-AFLP:

complementary DNA — amplified fragment length polymorphism

DEF:

differentially expressed fragment

EST-SSR:

expressed sequence tag — simple sequence repeat

Fv/Fm :

variable to maximum chlorophyll fluorescence (maximum potential quantum efficiency of photosystem 2)

HRM:

high resolution melting curve analysis

LG:

linkage group

QTL:

quantitative trait loci

RWC:

relative water content

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Authors and Affiliations

  1. Institute of Agriculture, Lithuanian Research Centre for Agriculture and Forestry, Instituto a.1, Akademija, LT-58344, Lithuania

    K. Jonavičienė, V. Paplauskienė, S. Lazauskas & G. Brazauskas

  2. Department of Genetics and Biotechnology, Faculty of Agricultural Sciences, Research Center Flakkebjerg, Aarhus University, Forsøgsvej 1, 4200, Slagelse, Denmark

    B. Studer & T. Asp

  3. Roche Diagnostics A/S, Industriholmen 59, 2650, Hvidovre, Denmark

    L. B. Jensen

Authors
  1. K. Jonavičienė
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  2. B. Studer
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  3. T. Asp
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  4. L. B. Jensen
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  5. V. Paplauskienė
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  6. S. Lazauskas
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  7. G. Brazauskas
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Correspondence to K. Jonavičienė.

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Acknowledgments: this study has been funded by the Research Council of Lithuania grant PBT-06 and by CIRIUS grant AM-DK-2009-LT-0265.

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Jonavičienė, K., Studer, B., Asp, T. et al. Identification of genes involved in a water stress response in timothy and mapping of orthologous loci in perennial ryegrass. Biol Plant 56, 473–483 (2012). https://doi.org/10.1007/s10535-012-0110-6

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  • DOI: https://doi.org/10.1007/s10535-012-0110-6

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