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Nucleotide polymorphism pattern and multiple maternal origin in Thinopyrum intermedium inferred by trnH-psbA sequences

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Biologia Plantarum

Abstract

Thinopyrum intermedium is an important species with potential utilization value in breeding of wheat. In this study, the non-coding intergenic region of trnH-psbA was investigated to assess the genetic diversity and infer the maternal origin within T. intermedium accessions. Eleven haplotypes were distinguished among the thirty-five accessions of T. intermedium. They showed a relatively low nucleotide diversity (π) of 0.00473 ± 0.00037 and a moderately high haplotype diversity (Hd) of 0.733 ± 0.061. In the phylogenetic analysis, all accessions of T. intermedium were positioned into two clades, which corresponded to the different diploid donors. These results suggested that there were two phylogenetically divergent maternal donors in T. intermedium.

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Abbreviations

CTAB:

cetyltrimethylammonium bromide

PCR:

polymerase chain reaction

bp:

base pair

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Acknowledgements

The authors are thankful to the Excellent Doctoral Dissertation Fund of Sichuan Agricultural University (YBPY0906), the National Natural Science Foundation of China (Nos. 30870154, 30901052, 30900087), and the Science and Technology Department and Education Department of Sichuan Province, China for the financial support.

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Authors and Affiliations

  1. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, P.R. China

    J. Zeng, X. Fan, L. -N. Sha, H. -Y. Kang, H. -Q. Zhang & Y. -H. Zhou

  2. College of Resources and Environment, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, P.R. China

    J. Zeng

  3. Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, P.R. China

    Y. -H. Zhou

  4. College of Biology and Science, Sichuan Agricultural University, Yaan, 625014, Sichuan, P.R. China

    J. Liu, X. -L. Wang & R. -W. Yang

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Correspondence to Y. -H. Zhou.

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Zeng, J., Fan, X., Sha, L.N. et al. Nucleotide polymorphism pattern and multiple maternal origin in Thinopyrum intermedium inferred by trnH-psbA sequences. Biol Plant 56, 254–260 (2012). https://doi.org/10.1007/s10535-012-0084-4

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  • DOI: https://doi.org/10.1007/s10535-012-0084-4

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