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Genetic diversity in important members of Cucurbitaceae using isozyme, RAPD and ISSR markers

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Biologia Plantarum

Abstract

Biochemical and molecular markers have been used on eleven species of Cucurbitaceae collected from lower Gangetic plains. Six enzyme systems were selected. Among 40 primers examined, 14 random amplified polymorphic DNA (RAPD) and 10 inter-simple sequence repeat (ISSR) primers were selected for the analysis. Generated RAPD (100) and ISSR (100) fragments showed high variations among the species. Jaccard similarity coefficients were used for the evaluation of pairwise genetic divergence; cluster analysis of the similarity matrices was performed to estimate interspecific diversity. Further, principal coordinate analysis was performed to evaluate the resolving power of the three marker systems to differenciate among the species.

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Abbreviations

ISSR:

inter-simple sequence repeat

PCR:

polymerase chain reaction

RAPD:

random amplified polymorphic DNA

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Acknowledgements

B. Sikdar is gratefully acknowledged to TWAS for financial support to the postdoctoral research.

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Authors and Affiliations

  1. Department of Genetic Engineering and Biotechnology, University of Rajsahi, Rajsahi, Bangladesh

    B. Sikdar

  2. Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, 700019, West Bengal, India

    M. Bhattacharya, A. Banerjee, E. Ghosh, B. Ghosh & S. C. Roy

  3. Department of Botany, Dinabandhu Mahavidyalaya, Bongaon, 743235, West Bengal, India

    A. Mukherjee

Authors
  1. B. Sikdar
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  2. M. Bhattacharya
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  3. A. Mukherjee
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  4. A. Banerjee
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  5. E. Ghosh
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  6. B. Ghosh
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  7. S. C. Roy
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Correspondence to S. C. Roy.

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Sikdar, B., Bhattacharya, M., Mukherjee, A. et al. Genetic diversity in important members of Cucurbitaceae using isozyme, RAPD and ISSR markers. Biol Plant 54, 135–140 (2010). https://doi.org/10.1007/s10535-010-0021-3

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  • DOI: https://doi.org/10.1007/s10535-010-0021-3

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