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Effect of 24-epibrassinolide on drought stress-induced changes in Chorispora bungeana

  • Brief Communication
  • Published:
Biologia Plantarum

Abstract

Brassinosteroids (BRs) have been proposed to increase the resistance of plants to drought stress. The effect of foliar application of 0.1 μM 24-epibrassinolide (EBR) on chlorophyll (Chl) content, photosystem 2 (PS 2) photochemistry, membrane permeability, lipid peroxidation, relative water content (RWC), proline content, and the antioxidant system in drought-stressed Chorispora bungeana plants was investigated. The results showed that polyethylene glycol (PEG) induced water stress decreased RWC, Chl content and variable to maximum Chl fluorescence ratio (Fv/Fm) less in plants pretreated with EBR than in non-pretreated plants. In addition, lipid peroxidation, measured in terms of malondialdehyde content, membrane permeability and proline content in drought-stressed plants were less increased in EBR pretreated plants, while antioxidative enzyme activities and reduced ascorbate and glutathione contents were more increased in EBR pretreated than in non-pretreated plants. These results suggested that EBR could improve plant growth under drought stress

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Abbreviations

APX:

ascorbate peroxidase

AsA:

reduced ascorbate

BRs:

brassinosteroids

CAT:

catalase

Chl:

chlorophyll

EBR:

24-epibrassinolide

EDTA:

ethylenediaminetetraacetic acid

EL:

electrolyte leakage

F0, Fm :

minimum and maximum fluorescence in dark-adapted leaves

Fv/Fm :

variable to maximum fluorescence ratio

GR:

glutathione reductase

GSH:

reduced glutathione

MDA:

malondialdehyde

PEG:

polyethylene glycol

PS 2:

photosystem 2

ROS:

reactive oxygen species

RWC:

relative water content

SOD:

superoxide dismutase

ΦPS2 :

the quantum yield of PS 2

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (grant No. 30900172), the National Outstanding Youth Foundation of China (No. 30625008), and the Major Project of Cultivating New Varieties of Transgenic Organisms (2009ZX08009-029B, 2008ZX08009-003)

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Authors and Affiliations

  1. Key Laboratory of Arid and Pasture Agroecology of Ministry of Education, School of Life Sciences, Lanzhou University, Lanzhou, 730000, Gansu, P.R. China

    Y. H. Li, Y. J. Liu, X. L. Xu, M. Jin, L. Z. An & H. Zhang

  2. Department of Life Sciences, Zhengzhou Normal University, Zhengzhou, 450044, Henan, P.R. China

    Y. H. Li

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  1. Y. H. Li
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  2. Y. J. Liu
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  3. X. L. Xu
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  4. M. Jin
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  5. L. Z. An
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  6. H. Zhang
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Correspondence to H. Zhang.

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Li, Y.H., Liu, Y.J., Xu, X.L. et al. Effect of 24-epibrassinolide on drought stress-induced changes in Chorispora bungeana . Biol Plant 56, 192–196 (2012). https://doi.org/10.1007/s10535-012-0041-2

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  • DOI: https://doi.org/10.1007/s10535-012-0041-2

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