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Indole-3-acetic acid accumulation during poplar rhizogenesis revealed by immunohistochemistry

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Biologia Plantarum

Abstract

Poplar hybrid 741 [Populus alba × (P. davidiana + P. simonii) × P. tomentosa] leaves were rooted within 8 d when cultured in vitro on 1/2 Murashige and Skoog (MS) medium. The spatial distribution of endogenous indole-3-acetic acid (IAA) in the rhizogenesis was investigated, using an immunohistochemical approach. In addition, the effect of 2,3,5-triiodobenzoic acid (TIBA) on IAA distribution was also analyzed. The results showed that a strong IAA signal was detected in the vascular bundles of the basal regions of the petioles 3 d after root induction. Furthermore, the signal in vascular bundles of the basal regions of the petioles was stronger than that of the middle regions of the petioles. Application of TIBA on lamina delayed both the accumulation of IAA in the vascular bundles and rhizogenesis. These data indicate that an endogenous IAA rise in vascular bundles is among the first signals leading to the rhizogenesis, and that it results from transportation of the hormone from the lamina of the leaf to the base of the petiole, rather than by in situ IAA generation.

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Abbreviations

6-BA:

6-benzyl aminopurine

BSA:

bovine serum albumin

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

EDC:

1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide

MS:

Murashige and Skoog

TBS:

Tris buffer solution

TIBA:

2,3,5-triiodobenzoic acid

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Authors and Affiliations

  1. School of Forestry, Beijing Forestry University, Beijing, 100083, P.R. China

    N. G. Dong & W. L. Yin

  2. State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, P.R. China

    N. G. Dong, Y. Gao & D. Pei

Authors
  1. N. G. Dong
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  2. W. L. Yin
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  3. Y. Gao
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  4. D. Pei
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Corresponding author

Correspondence to D. Pei.

Additional information

Acknowledgements: We sincerely thanked Dr. Mingyong Chen (College of Veterinary Medicine, China Agricultural University, China) and Dr. Zhixia Hou (School of Forestry, Beijing Forestry University, China) for their kind help with experiment methods and the equipment. This work was supported by the National Natural Science Foundation of China (30671436) and National Science and Technology Infrastructure Program of China (2006BAD01A1730).

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Dong, N.G., Yin, W.L., Gao, Y. et al. Indole-3-acetic acid accumulation during poplar rhizogenesis revealed by immunohistochemistry. Biol Plant 56, 581–584 (2012). https://doi.org/10.1007/s10535-011-0230-4

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  • DOI: https://doi.org/10.1007/s10535-011-0230-4

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