Abstract
The production and characterization of high-affinity monoclonal antibodies suitable for the radio- and enzymeimmunoassay of the endogenous plant growth regulator, indole-3-acetic acid (IAA), is reported. Hybridomas were produced by fusion of NS 1 myeloma cells with spleen cells from Balb/c mice immunized with IAA-bovine serum albumin conjugates. From an initial collection of 158 wells containing cells secreting monoclonal antibodies against IAA, seven were used to derive cell clones. Three of these are described here. They secrete immunoglobulin (IgG2a or IgG2b) of high affinity and specificity for IAA methyl ester and can be used to quantite picogram amounts of this compound in plant extracts by radio- and enzymeimmunoassay.
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Abbreviations
- EIA:
-
enzyme immunoassay
- HPLC:
-
high-performance liquid chromatography
- IAA:
-
indole-3-acetic acid
- mAB:
-
monoclonal antibodies
- RIA:
-
radioimmunoassay
References
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I=Mertens et al. (1983)
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Mertens, R., Eberle, J., Arnscheidt, A. et al. Monoclonal antibodies to plant growth regulators. II. Indole-3-acetic acid. Planta 166, 389–393 (1985). https://doi.org/10.1007/BF00401177
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DOI: https://doi.org/10.1007/BF00401177