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Clonal propagation of Zephyranthes grandiflora using bulbs as explants

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  • Published:
Biologia Plantarum

Abstract

Zephyr lily (Zephyranthes grandiflora), an important ornamental plant has been micropropagated in vitro after controlling microbial contamination by a pretreatment with 0.2 % Bavistin and 0.1 % Pantomycin for 4 h before final sterilization with 0.1 % mercuric chloride. In 67 % of the sterile cultures, 11 shoots on average were regenerated directly from basal half of bulb scales in Murashige and Skoog (MS) medium containing 3 % sucrose and 2 mg dm−3 benzylaminopurine (BAP). Shoots emerged in bunches on a basal achlorophyllous bulbous part. Combination of 2 mg dm−3 BAP with 1 mg dm−3 gibberellic acid (GA3) enhanced shoot growth. Stout roots (maximum of 5–6 per shoot) were developed in presence of 1 mg dm−3 indole-3-butyric acid (IBA). Micro-bulbs showed potential of regeneration and could be used as secondary explants. The morphologically identical plants derived by in vitro propagation were genetically identical as shown by PCR based ISSR marker analysis of genomic DNA.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

BAP:

6-benzylaminopurine

dNTPs:

deoxyribonucleotide triphosphate

EtBr:

ethidium bromide

GA3:

gibberellic acid-3

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

ISSR:

inter simple sequence repeat

MS:

Murashige and Skoog

NAA:

1-naphthalene acetic acid

PCR:

polymerase chain reaction

TBE:

tris borate EDTA

References

  • Azadi, P., Khosh-Khui, M.: Micropropagation of Lillium ledebourii (Baker) Boiss as affected by plant growth regulator, sucrose concentration, harvesting season and cold treatments. — Electron. J. Biotechnol. 10: 582–597, 2007.

    Article  Google Scholar 

  • Chow, Y.N., Fraser, T.W., Selby, C., Harvey, B.M.R.: Basal plate tissue in Narcissus bulbs and in shoot clump cultures: its structure and role in organogenic potential of single leaf cultures. — Ann. Bot. 71: 437–443, 1993.

    Article  Google Scholar 

  • Dellaporta, S.L., Woods, J., Hicks, J.B.: A plant DNA minipreparation. Version 2. — Plant mol. Biol. Rep. 1: 19–22, 1993.

    Article  Google Scholar 

  • Feyissa, T., Welander, M., Negash, L.: Genetic stability, ex vitro rooting and gene expression studies in Hagenia abyssinica. — Biol. Plant. 51: 15–21, 2007.

    Article  CAS  Google Scholar 

  • Han, B.H., Suh, E.J., Choi, S.L., Yae, B.W., Yu, H.J., Goo, D.H.: Stimulation of in vitro bulbet growth by the addition of liquid medium in Lilium oriental hybrid ‘Casablanca’. — J. Plant Biotechnol. 6: 241–246, 2004.

    Google Scholar 

  • Huang, C.W., Hiroshi, O., Uemoto, S.: Importance of two scales in propagating Hipperastrum hygridum by twin scaling. — Sci. Hort. 42: 141–149, 1990.

    Article  Google Scholar 

  • Huang, W.J., Ning, G.G., Bao, M.Z.: Determination of genetic stability of long-term micropropagated plantlets of Platanus acerifolia using ISSR markers. — Biol. Plant. 53: 159–163, 2009.

    Article  Google Scholar 

  • Joshi, P., Dhawan, V.: Assessment of genetic fidelity of micropropagated Swerita chiraytia plantlets by ISSR marker assay. — Biol. Plant. 51: 22–26, 2007.

    Article  CAS  Google Scholar 

  • Kumar, S., Kashyap, M., Sharma, D.R.: In vitro regeneration and bulblet growth from lily bulbscale explants as affected by retardants, sucrose and irradiance. — Biol. Plant. 49: 629–632, 2005.

    Article  Google Scholar 

  • Liu, L., Burger, D.W.: In vitro propagation of Easter lily from pedicels. — Hort. Sci. 21: 1437–1438, 1986.

    CAS  Google Scholar 

  • Mukhopadhyay, M.J., Mukhopadhyay, S., Sen, S.: In vitro propagation of Iphigenia indica, an alternative source of colchicines. — Plant Cell Tissue Organ Cult. 69: 101–104, 2002.

    Article  CAS  Google Scholar 

  • Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. — Physiol. Plant. 15: 473–497, 1962.

    Article  CAS  Google Scholar 

  • Ogden, S. (ed.): Garden Bulbs for the South. — Timber Press, Portland 2007.

    Google Scholar 

  • Ray, A., Bhattacharya, S.: Regeneration of genetically uniform Boerhaavia diffusa by in vitro culture of nodal explants and synthetic seeds. — Int. J. Dev. Biol. 2: 123–127, 2008.

    Google Scholar 

  • Saker, M.M., Adawy, S.S., Mohamed, A.A., El-Itriby, H.A.: Monitoring of cultivar identity in tissue culture-derived date palms using RAPD and AFLP analysis. — Biol. Plant. 50: 198–204, 2006.

    Article  CAS  Google Scholar 

  • Sharma, K.D., Rathour, R., Sharma, R., Goel, S., Sharma, T.R., Singh, B.M.: In vitro cormlet development in Crocus sativus. — Biol. Plant. 52: 709–712, 2008.

    Article  CAS  Google Scholar 

  • Shivakumar, G., Krishnamurthy, K.V.: In vitro organogenetic responses of Gloriosa superba. — Russ. J. Plant Physiol. 51: 713–721, 2004.

    Article  Google Scholar 

  • Smith, R.H., Burrows, J., Kurten, K.: Challenges associated with micropropagation of Zephyranthes and Hippesatrum sp. (Amaryllidaceae). — In Vitro cell. dev. Biol. Plant 35: 281–282, 1999.

    Article  Google Scholar 

  • Xing, Y., Yu, Y., Luo, X., Zhang, J.-N., Zhao, B., Guo, Y.-D.: High efficiency organogenesis and analysis of genetic stability of the regenerants in Solanum melongena. — Biol. Plant. 54: 213–236, 2010.

    Article  Google Scholar 

  • Ye, S.H., Chen, S., Zhang, F., Wang, W., Tian, Q., Liu, J.Z., Chen, F., Bao, J.K.: Transgenic tobacco expressing Zephyranthes grandiflora agglutinin confers enhanced resistance to aphids. — Appl Biochem Biotechnol. 158: 615–30, 2009.

    Article  CAS  PubMed  Google Scholar 

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Acknowledgement

Authors are thankful to Mr. Jadav Ghosh for technical assistance, and Director of Bose Institute for providing necessary facilities.

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Authors and Affiliations

  1. Medicinal Plant Laboratory, Department of Botany, Bose Institute, 93/1 A.P.C. Road, Kolkata, 700009, India

    M. Gangopadhyay & S. Bhattacharya

  2. Department of Bioscience and Biotechnology, Banasthali Vidypith, Rajasthan, 304022, India

    D. Chakraborty

  3. Department of Pharmaceutical Technology, Jadavpur University, Kolkata, 700032, India

    S. Dewanjee

Authors
  1. M. Gangopadhyay
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  2. D. Chakraborty
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  3. S. Dewanjee
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  4. S. Bhattacharya
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Correspondence to M. Gangopadhyay.

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Gangopadhyay, M., Chakraborty, D., Dewanjee, S. et al. Clonal propagation of Zephyranthes grandiflora using bulbs as explants. Biol Plant 54, 793–797 (2010). https://doi.org/10.1007/s10535-010-0145-5

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  • DOI: https://doi.org/10.1007/s10535-010-0145-5

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