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Heat tolerance in rice mutants is associated with reduced accumulation of reactive oxygen species

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Biologia Plantarum

Abstract

Four mutants induced by ethylmethane sulphonate (N22-H-dgl56, N22-H-dgl101, N22-H-dgl162 and N22-H-dgl219) with conspicuous dark green leaves were identified in the drought and heat-tolerant rice cultivar Nagina22 (N22), when screened under prolonged drought and heat conditions in field. During dark-induced senescence, these mutants maintained higher chlorophyll and carotenoid contents, and photochemical efficiency of photosystem 2 in comparison with N22. Following heat treatment, these mutants accumulated less reactive oxygen species (assayed by histochemical staining for H2O2 and superoxide radicals) and maintained higher chlorophyll content than N22.

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Abbreviations

dgl :

dark green leaf mutant

DAB:

diaminobenzidine

EMS:

ethylmethane sulphonate

NBT:

nitroblue tetrazolium

ROS:

reactive oxygen species

SOD:

superoxide dismutase.

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Acknowledgements

We thank Department of Biotechnology, Govt. of India (BT/PR9264/AGR/02/406(04)/2007) for financial support and Dr. R. P. Sharma, NRCPB, Delhi, Coordinator, DBT Network Project on Nagina22 mutants and Dr. S.R. Voleti for useful discussions. We thank the other Principal Investigators in the network project: Dr. A.K. Singh, IARI, New Delhi; Dr. T. Mohapatra, NRCPB, New Delhi for generating mutant population and Dr. K. Singh, PAU, Ludhiana; Dr. S. Robin, TNAU, Coimbatore and Dr. M. Sheshshayee, UAS, Bangalore. The mutant lines have been deposited in the mutant bank of the network project. We thank G. Ashok Reddy for field assistance. We thank the instrumentation unit and the central facility of CCMB, Hyderabad for their help.

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Authors and Affiliations

  1. Directorate of Rice Research, Rajendra Nagar, Hyderabad, 500030, India

    M. Panigrahy, S. Neelamraju & D. Nageswara Rao

  2. Centre for Cellular and Molecular Biology, Hyderabad, 500007, India

    R. Ramanan

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  1. M. Panigrahy
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  2. S. Neelamraju
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  3. D. Nageswara Rao
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  4. R. Ramanan
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Correspondence to R. Ramanan.

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Panigrahy, M., Neelamraju, S., Rao, D.N. et al. Heat tolerance in rice mutants is associated with reduced accumulation of reactive oxygen species. Biol Plant 55, 721–724 (2011). https://doi.org/10.1007/s10535-011-0175-7

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  • DOI: https://doi.org/10.1007/s10535-011-0175-7

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