Skip to main content
SpringerLink
Log in

Development of novel chloroplast microsatellite markers for Cucumis from sequence database

  • Brief Communication
  • Published:
Biologia Plantarum

Abstract

The development of chloroplast microsatellite (cpSSR) markers in Cucumis species and analysis of their polymorphism and transferability were reported. Fifteen microsatellite markers, represented by mononucleotide repeats, were developed from the complete sequence of Cucumis sativus chloroplast genome. Intraspecific variation was successfully detected in C. sativus and C. melo and revealed mean 1.6 and 1.9 alleles per cpSSR locus, respectively. With the exception of two exon region-located cpSSR markers being monomorphic, each of the others amplified polymorphic fragments in C. sativus or C. melo. A total of 34 polymorphic loci were detected with these cpSSR markers in the two species. Transferability of the newly developed cpSSR markers was checked on an additional set of 41 Cucurbitaceae accessions (belonging to 12 different species), and except for two markers with no amplification in Cucurbita maxima, the others could be transferable to all the accessions tested. Of the 15 cpSSR markers, 14 markers generated fragments with expected band sizes and 13 markers detected interspecific polymorphism among the accessions. Intraspecific polymorphism was also observed within four Cucurbitaceae species excluding C. sativus and C. melo.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Abbreviations

SSR:

microsatellite or simple sequence repeat

cpSSR:

chloroplast microsatellite

RFLP:

restriction fragment length polymorphism

CTAB:

cetyltrimethylammonium bromide

References

  • Birky, C.W.: Evolution and population genetics of organelle genes: mechanisms and models. — In: Gottlieb, L.D., Jain, S.K. (ed.): Plant Evolutionary Biology. Pp. 23–53. Chapman & Hall, London 1988.

    Google Scholar 

  • Bryan, G.J., McNicoll, J., Ramsay, G., Meyer, R.C., De Jong, W.S.: Polymorphic simple sequence repeat markers in chloroplast genomes of Solanaceous plants. — Theor. appl. Genet. 99: 859–867, 1999.

    Article  CAS  Google Scholar 

  • Danin-Poleg, Y., Reis, N., Tzuri, G., Katzir, N.: Development and characterization of microsatellite markers in Cucumis. — Theor. appl. Genet. 102: 61–72, 2001.

    Article  CAS  Google Scholar 

  • Ishii, T., McCouch, S.R.: Microsatellites and microsynteny in the chloroplast genomes of Oryza and eight other Gramineae species. — Theor. appl. Genet. 100: 1257–1266, 2000.

    Article  CAS  Google Scholar 

  • Kong, Q., Xiang, C., Yu, Z.: Development of EST-SSRs in Cucumis sativus from sequence database. — Mol. Ecol. Notes 6: 1234–1236, 2006.

    Article  CAS  Google Scholar 

  • Kong, Q., Xiang, C., Yu, Z., Zhang, C., Liu, F., Peng, C., Peng, X.: Mining and charactering microsatellites in Cucumis melo expressed sequence tags from sequence database. — Mol. Ecol. Notes 7: 281–283, 2007.

    Article  CAS  Google Scholar 

  • McGrath, S., Hodkinson, T.R., Salamin, N., Barth, S.: Development and testing of novel chloroplast microsatellite markers for Lolium perenne and other grasses (Poaceae) from de novo sequencing and in silico sequences. — Mol. Ecol. Notes 6: 449–452, 2006.

    Article  CAS  Google Scholar 

  • Murray, M.G., Thompson, W.F.: Rapid isolation of high molecular weight plant DNA. — Nucl. Acids Res. 10: 4321–4325, 1980.

    Article  Google Scholar 

  • Nei, M. (ed.): Molecular Evolutionary Genetics. — Columbia University Press, New York 1987.

  • Provan, J., Corbett, G., McNicol, J.W, Powell, W.: Chloroplast DNA variability in wild and cultivated rice (Oryza spp.) revealed by polymorphic chloroplast simple sequence repeats. — Genome 40: 104–410, 1997.

    Article  CAS  PubMed  Google Scholar 

  • Provan, J., Powell, W., Hollingsworth, P.M.: Chloroplast microsatellites: new tools for studies in plant ecology and evolution. — Trends Ecol. Evol. 16: 142–147, 2001.

    Article  PubMed  Google Scholar 

  • Provan, J., Russell, J.R., Booth, A., Powell, W.: Polymorphic chloroplast simple sequence repeat primers for systematic and populations studies in the genus Hordeum. — Mol. Ecol. 8: 505–511, 1999.

    Article  CAS  PubMed  Google Scholar 

  • Skálová, D., Dziechciarková, M., Lebeda, A., Křístková, E., Navrátilová, B.: Interspecific hybridization of Cucumis anguria and C. zeyheri via embryo-rescue. — Biol. Plant. 52: 775–778, 2008.

    Article  Google Scholar 

  • Watcharawongpaiboon, N., Chunwongse, J.: Development and characterization of microsatellite markers from an enriched genomic library of cucumber (Cucumis sativus). — Plant Breed. 127: 74–81, 2008.

    Google Scholar 

  • Weising, K., Gardner, R.C.: A set of conserved PCR primers for the analysis of simple sequence repeat polymorphisms in chloroplast genomes of dicotyledonous angiosperms. — Genome 42: 9–19, 1999.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Horticulture, Henan Agricultural University, Zhengzhou, 450002, P.R. China

    J. -B. Hu & J. -W. Li

  2. College of Horticulture, Northeast Agricultural University, Harbin, 150030, P.R. China

    X. -Y. Zhou

Authors
  1. J. -B. Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. X. -Y. Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. -W. Li
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J. -W. Li.

Additional information

Acknowledgements: This work is supported by a grant from the National High Technology Research and Development Program of China (863 Program, No. 2007AA10Z100). The authors are grateful to Prof. W.G. Liu and Dr. S.J. Zhao, Zhengzhou Fruit Institute, Chinese Academy of Agriculture Science, for their assistance in the laboratory work on this project.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hu, J.B., Zhou, X.Y. & Li, J.W. Development of novel chloroplast microsatellite markers for Cucumis from sequence database. Biol Plant 53, 793–796 (2009). https://doi.org/10.1007/s10535-009-0146-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10535-009-0146-4

Additional key words

Search

Navigation