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Physiological effects of exogenous nitric oxide on Brassica juncea seedlings under NaCl stress

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Biologia Plantarum

Abstract

The study was conducted to investigate the physiological effects of exogenous NO on potherb mustard (Brassica juncea Coss.) seedlings under salt stress. The plants were grown in Hogland nutrient solution for 15 d and treated with 150 mM NaCl, NO donor sodium nitropruside (SNP) and NO scavenger methylene blue (MB-1) for 4 d. The NaCl stress increased superoxide dismutase, peroxidase and ascorbate peroxidase activities and malondialdehyde (MDA) and free proline contents, and decreased soluble protein content. However, the application of exogenous NO limited the production of MDA and free proline, while markedly promoted SOD, POD and APX activity.

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Abbreviations

APX:

ascorbate peroxidase

MB-1:

methylene blue

MDA:

malondialdehyde

POD:

peroxidase

PTIO:

2-(4-carboxyphenyl-4,4,5,5-tetramethylimid-azoline-1-oxyl-3-oxide

SOD:

superoxide dismutase

SNS:

sodium nitropruside

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Acknowledgements

This work was supported by National Natural Science Foundation of Hubei province (Grant No. 2008CDB087), Chinese National Basic Research and Development Program (2006CB101607), 863-Hi-techresearch and Development Program of China (2006AA10Z1E4).

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Authors and Affiliations

  1. College of Life Sciences, Jianghan University, Wuhan, 430056, P.R. China

    C. -L. Zeng & Y. Zhou

  2. Institute of Oil Crop Research, Chinese Academy of Agricultural Sciences, Wuhan, 430078, P.R. China

    C. -L. Zeng & X. -M. Wu

  3. College of Arts and Sciences, Jianghan University, Wuhan, 430056, P.R. China

    L. Liu

  4. College of Plant Science and Technology, Huangzhong Agricultural University, Wuhan, 430073, P.R. China

    B. -R. Wang

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  1. C. -L. Zeng
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  2. L. Liu
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  4. X. -M. Wu
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  5. Y. Zhou
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Correspondence to C. -L. Zeng.

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Zeng, C.L., Liu, L., Wang, B.R. et al. Physiological effects of exogenous nitric oxide on Brassica juncea seedlings under NaCl stress. Biol Plant 55, 345–348 (2011). https://doi.org/10.1007/s10535-011-0051-5

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  • DOI: https://doi.org/10.1007/s10535-011-0051-5

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