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Response of antioxidant enzymes to high NaCl concentration in different salt-tolerant plants

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Biologia Plantarum

Abstract

The effects of NaCl on the H2O2 content and the activities of catalase (CAT) and superoxide dismutase (SOD) were studied in diverse group of plants, such as a unicellular alga, Chlorella sp., an aquatic macrophyte, Najas graminea, and a mangrove plant, Suaeda maritima, all showing high tolerance to NaCl. Significant accumulation of H2O2 was observed in all the tested plants upon their exposure to 255 mM NaCl. The activity of both CAT and SOD increased significantly in response to the NaCl treatment. Growing the plants in presence of 255 mM NaCl also resulted in the synthesis of new isoforms of both CAT and SOD.

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Abbreviations

APX:

ascorbate peroxidase

CAT:

catalase

H2O2 :

hydrogen peroxide

KCN:

potassium ferricyanide

NBT:

nitroblue tetrazolium

O2 •- :

superoxide

•OH:

hydroxyl radical

PAGE:

polyacrylamide gel electrophoresis

ROS:

reactive oxygen species

SOD:

superoxide dismutase

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Acknowledgements

The authors are thankful to Dr. B. Ravindran, the director of the Institute of Life Sciences, for providing the laboratory facilities for the work, and to DBT, New Delhi for financial support as extra-mural grant.

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Authors and Affiliations

  1. Institute of Life Sciences, Nalco Square, Bhubaneswar, 751023, Orissa, India

    S. Mallik, B. B. Sahu & B. P. Shaw

  2. Department of Biotechnology, Berhampur University, Berhampur, 760007, Orissa, India

    M. Nayak & A. K. Panigrahi

Authors
  1. S. Mallik
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  2. M. Nayak
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  3. B. B. Sahu
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  4. A. K. Panigrahi
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  5. B. P. Shaw
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Correspondence to B. P. Shaw.

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Mallik, S., Nayak, M., Sahu, B.B. et al. Response of antioxidant enzymes to high NaCl concentration in different salt-tolerant plants. Biol Plant 55, 191–195 (2011). https://doi.org/10.1007/s10535-011-0029-3

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  • DOI: https://doi.org/10.1007/s10535-011-0029-3

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