Abstract
The influence of combined and individually applied drought and heat stress was studied in two wheat (Triticum aestivum L.) cultivars: resistant cv. Katya and susceptible cv. Sadovo. Relative water content decreased and electrolyte leakage increased due to individual and combined application of both stresses. Initial heat shock protein profile has been outlined via SDS electrophoresis of leaf extracts. The results obtained were confirmed by immunoblotting with anti-HSP70 monoclonal antibodies, anti-HSP110 polyclonal antibodies and anti-α β-crystalline polyclonal antibodies. The effect of simultaneously applied water stress and heat shock resembled the alterations in protein expression provoked only by water stress and differed significantly from the changes occurring after the individual application of heat stress.
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Abbreviations
- D:
-
drought stress
- DH:
-
combined drought and heat stress
- EDTA:
-
ethylendiaminetetracetic acid
- H:
-
heat stress
- HSP:
-
heat shock protein
- PMSF:
-
phenylmethanesulfonyl fluoride
- RLS and RSS:
-
Rubisco large and small subunits, respectively
- SDS-PAGE:
-
sodium dodecyl sulfate polyacrylamide gel electrophoresis
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Acknowledgements
This study was supported financially by the SCOPES program of the Swiss National Science Foundation (project DILPA-JRP-IB73AO-111142/1) and the Ministry of Education and Science of the Republic of Bulgaria (Contract No. CC1503). The authors are grateful to Dr. V. Vassileva for her assistance in the conductivity measurement analyses. They would also like to acknowledge the efforts of Dr. M. Stamenova who provided the α β-crystalline antibody. Thanks are extended to Mrs. B. Juperlieva- Mateeva, A. Kostadinova and I. Anders for their technical assistance.
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Grigorova, B., Vaseva, I., Demirevska, K. et al. Combined drought and heat stress in wheat: changes in some heat shock proteins. Biol Plant 55, 105–111 (2011). https://doi.org/10.1007/s10535-011-0014-x
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DOI: https://doi.org/10.1007/s10535-011-0014-x