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Starch synthase activity and heat shock protein in relation to thermal tolerance of developing wheat grains

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Biologia Plantarum

Abstract

Wheat (Triticum aestivum L.) cvs. HD 2285 (relatively tolerant) and WH 542 (susceptible) were exposed to ambient and elevated temperature (3–4 °C higher) in open top chambers during post anthesis period. The grain yield components were determined at the time of maturity. In order to elucidate the basis of differential tolerance of these cultivars, the excised developing grains (20 d after anthesis) of ambient grown plants were incubated at 15, 25, 35 and 45 °C for 2 h and then analysed for the activities of soluble starch synthase (SSS), granule bound starch synthase (GBSS), kinetic parameters of SSS and content of heat shock protein (HSP 100). The elevated temperature during grain development significantly decreased grain growth in WH 542 whereas no such decrease was observed in HD 2285. High temperature tolerance of HD 2285 was found to be associated with higher catalytic efficiency (Vmax/Km) of SSS at elevated temperature and higher content of HSP 100.

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Abbreviations

ADPG:

adenosine diphosphate glucose

DAA:

days after anthesis

GBSS:

granule bound starch synthase

HSP:

heat shock protein

OTC:

open top chamber

S:

heat susceptibility index

SSS:

soluble starch synthase

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Authors and Affiliations

  1. Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi, 110012, India

    K. V. Sumesh, P. Sharma-Natu & M. C. Ghildiyal

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  1. K. V. Sumesh
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  2. P. Sharma-Natu
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  3. M. C. Ghildiyal
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Correspondence to M. C. Ghildiyal.

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Sumesh, K.V., Sharma-Natu, P. & Ghildiyal, M.C. Starch synthase activity and heat shock protein in relation to thermal tolerance of developing wheat grains. Biol Plant 52, 749–753 (2008). https://doi.org/10.1007/s10535-008-0145-x

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  • DOI: https://doi.org/10.1007/s10535-008-0145-x

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