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Effect of osmotic stress and sodium nitroprusside pretreatment on proline metabolism of wheat seedlings

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Biologia Plantarum

Abstract

Effect of osmotic stress and sodium nitroprusside (SNP, NO donor) pretreatment on growth and proline metabolism of wheat seedlings was investigated. Polyethylene glycol 6000 treatment for 2, 4 and 6 d could be termed as mild, moderate and severe stress, respectively, according to decrease in the relative water content. Severe osmotic stress significantly decreased the growth and photochemical efficiency, and increased proline content due to activation of its synthesis. 0.2 mM SNP pretreatment enhanced growth of wheat seedlings, increased variable to maximum fluorescence ratio (Fv/Fm) and fluorescence yield, while decreased proline content. However, 2 mM SNP retarded the seedlings growth and chlorophyll a fluorescence, and increased proline accumulation. Our results showed that NO might be involved in the regulation of osmotic stress in a concentration-dependent manner.

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Abbreviations

ABA:

abscisic acid

P5C:

Δ-pyrroline-5-carboxylate

P5CS:

P5C synthetase

PEG:

polyethylene glycol

RWC:

relative water content

SNP:

sodium nitroprusside

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Authors and Affiliations

  1. Chengdu Institute of Biology, Chinese Academy of Sciences, P.O.Box 416, Chengdu, 610041, P.R. China

    Y. Lei, C. Yin, J. Ren & C. Li

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  1. Y. Lei
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  2. C. Yin
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  3. J. Ren
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  4. C. Li
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Correspondence to C. Li.

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Lei, Y., Yin, C., Ren, J. et al. Effect of osmotic stress and sodium nitroprusside pretreatment on proline metabolism of wheat seedlings. Biol Plant 51, 386–390 (2007). https://doi.org/10.1007/s10535-007-0082-0

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  • DOI: https://doi.org/10.1007/s10535-007-0082-0

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