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Relationships among Leymus species assessed by RAPD markers

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Biologia Plantarum

Abstract

The DNA genetic diversity of 40 accessions of genus Leymus was analyzed by random amplified polymorphic DNA (RAPD) markers. A total of 352 products were amplified by 34 10-mer arbitrary primers, among which 337 products (95.74 %) were found to be polymorphic. 5–14 polymorphic bands were amplified by each polymorphic primer, with an average of 9.91 bands. The data of 352 RAPD bands were used to generate Jaccard’s similarity coefficients and to construct a dendrogram by means of UPGMA. Great genetic diversity in genus Leymus was observed, the genetic diversity among the different species more abundant than that of the different accessions, and the different accessions in a species or the species from the same areas were clustered together.

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Abbreviations

RAPD:

random amplified polymorphic DNA

UPGMA:

unweighed pair-group method analysis

SHAN:

sequential, hierarchical, agglomerative, and nested clustering

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Authors and Affiliations

  1. College of Biology and Science, Sichuan Agricultural University, Yaan, 625014, Sichuan, P.R. China

    R. W. Yang, C. B. Ding & L. Zhang

  2. Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan, 611830, Sichuan, P.R. China

    R. W. Yang, Y. H. Zhou & Y. L. Zheng

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  1. R. W. Yang
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  2. Y. H. Zhou
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  3. C. B. Ding
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  4. Y. L. Zheng
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  5. L. Zhang
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Correspondence to Y. H. Zhou.

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Yang, R.W., Zhou, Y.H., Ding, C.B. et al. Relationships among Leymus species assessed by RAPD markers. Biol Plant 52, 237–241 (2008). https://doi.org/10.1007/s10535-008-0052-1

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  • DOI: https://doi.org/10.1007/s10535-008-0052-1

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