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Linear fusigen as the major hydroxamate siderophore of the ectomycorrhizal Basidiomycota Laccaria laccata and Laccaria bicolor

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Abstract

A screening for siderophores produced by the ectomycorrhizal fungi Laccaria laccata and Laccaria bicolor in synthetic low iron medium revealed the release of several different hydroxamate siderophores of which four major siderophores could be identified by high resolution mass spectrometry. While ferricrocin, coprogen and triacetylfusarinine C were assigned as well as other known fungal siderophores, a major peak of the siderophore mixture revealed an average molecular mass of 797 for the iron-loaded compound. High resolution mass spectrometry indicated an absolute mass of m/z = 798.30973 ([M + H]+). With a relative error of Δ = 0.56 ppm this corresponds to linear fusigen (C33H52N6O13Fe; MW = 797.3). The production of large amounts of linear fusigen by these basidiomycetous mycorrhizal fungi may possibly explain the observed suppression of plant pathogenic Fusarium species. For comparative purposes Fusarium roseum was included in this study as a well known producer of cyclic and linear fusigen.

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Acknowledgments

The dikaryotic parental strain S238N and the sib-monokaryotic S238N-H82 of L. bicolor (Maire) P.D. Orton were kindly provided by F. Martin, INRA-Nancy, F-54280 Champenoux.

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Authors and Affiliations

  1. Department of Microbiology, University of Innsbruck, Technikerstr. 25, 6020, Innsbruck, Austria

    Kurt Haselwandter & Gerlinde Häninger

  2. Institute of Pharmacy, Pharmacognosy, University of Innsbruck, Innrain 80-82, 6020, Innsbruck, Austria

    Markus Ganzera

  3. Division of Molecular Biology/Biocenter, Innsbruck Medical University, Innrain 80-82, 6020, Innsbruck, Austria

    Hubertus Haas

  4. Institute of Organic Chemistry, University of Tübingen, Auf der Morgenstelle 18, 72076, Tübingen, Germany

    Graeme Nicholson

  5. Institute of Microbiology and Biotechnology, University of Tübingen, 72076, Tübingen, Germany

    Günther Winkelmann

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  1. Kurt Haselwandter
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  2. Gerlinde Häninger
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  3. Markus Ganzera
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  4. Hubertus Haas
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Correspondence to Günther Winkelmann.

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Haselwandter, K., Häninger, G., Ganzera, M. et al. Linear fusigen as the major hydroxamate siderophore of the ectomycorrhizal Basidiomycota Laccaria laccata and Laccaria bicolor . Biometals 26, 969–979 (2013). https://doi.org/10.1007/s10534-013-9673-8

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