Abstract
After analysis using HPLC and electronic ion spray mass spectroscopy, the purified siderophore produced by the marine-derived Aureobasidium pullulans HN6.2 was found to be fusigen. The purified desferric fusigen still had strong inhibition of growth of the pathogenic Vibrio anguillarum while the fusigen chelated by Fe3+ lost the ability to inhibit the growth of the pathogenic bacterium. The added iron in the medium repressed expression of the hydroxylase gene encoding ornithine N5-oxygenase that catalyzes the N5-hydroxylation of ornithine for the first step of siderophore biosynthesis in the yeast cells while expression of the hydroxylase gene in the yeast cells grown in the medium plus ornithine was enhanced.
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This work was supported by grant 30771645 from National Natural Science Foundation of China.
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Wang, W., Chi, Z., Liu, G. et al. Chemical and biological characterization of siderophore produced by the marine-derived Aureobasidium pullulans HN6.2 and its antibacterial activity. Biometals 22, 965–972 (2009). https://doi.org/10.1007/s10534-009-9248-x
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DOI: https://doi.org/10.1007/s10534-009-9248-x