Abstract
A bacterium growing on pyrazine-2-carboxylate broth was isolated, purified and identified as a strain of Stenotrophomonas sp. based on polyphasic taxonomic analyses and designated as strain HCU1. 16S rRNA gene sequence of strain HCU1 showed 98.7% sequence similarity with the type strain of Stenotrophomonas maltophilia belonging to Gammaproteobacteria. Growth of strain HCU1 was demonstrated when pyrazine-2-carboxylate was used as a sole source of nitrogen. Ring reduction of pyrazine-2-carboxylate was shown as increase in absorbance at 268 nm and the reduced product was confirmed as 1,2,5,6-tetrahydropyrazine-2-carboxylate, while a ring opened product, 2-amino-2-hydroxy-3-(methylamino) propanoic acid (with a loss in carbon atom), indicated a reductive degradation of pyrazine-2-carboxylate by strain HCU1.
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The infrastructural support provided by UGC-SAP and DST-FIST is acknowledged. KSR thank the UGC for UGC-SRF.
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Supplementary Fig 1: 1H (A) and 13C (B) NMR spectrum of the metabolite A1. Supplementary Fig 2: 1H (A), amplified 1H (B) and 13C NMR spectrum of metabolite B1. (PDF 436 kb)
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Rajini, K.S., Sasikala, C. & Ramana, C.V. Reductive degradation of pyrazine-2-carboxylate by a newly isolated Stenotrophomonas sp. HCU1. Biodegradation 21, 801–813 (2010). https://doi.org/10.1007/s10532-010-9345-0
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DOI: https://doi.org/10.1007/s10532-010-9345-0