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Mycoparasitism studies of Trichoderma species against three phytopathogenic fungi: evaluation of antagonism and hydrolytic enzyme production

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Abstract

Trichoderma spp. are used for biocontrol of several plant pathogens. However, their efficient interaction with the host needs to be accompanied by production of secondary metabolites and cell wall-degrading enzymes. Three parameters were evaluated after interaction between four Trichoderma species and plant-pathogenic fungi: Fusarium solani, Rhizoctonia solani and Sclerotinia sclerotiorum. Trichoderma harzianum and T. asperellum were the most effective antagonists against the pathogens. Most of the Trichoderma species produced toxic volatile metabolites, having significant effects on growth and development of the plant pathogens. When these species were grown in liquid cultures with cell walls from these plant pathogens, they produced and secreted β-1,3-glucanase, NAGAse, chitinase, acid phosphatase, acid proteases and alginate lyase.

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Acknowledgments

This work was funded by FINEP (Research and Projects Financing) and the National Council for Scientific and Technological Development (CNPq) and The State of Goiás Research Foundation (FAPEGO). T.F.Q was supported by a scholarship from CNPq. Dr. R. Pogue for reading this manuscript.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Federal University of Goiás (ICB), Goiânia, GO, 74001-970, Brazil

    Thiago Fernandes Qualhato, Fabyano Alvares Cardoso Lopes, Andrei Stecca Steindorff, Renata Silva Brandão, Rosália Santos Amorim Jesuino & Cirano José Ulhoa

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  1. Thiago Fernandes Qualhato
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  2. Fabyano Alvares Cardoso Lopes
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  3. Andrei Stecca Steindorff
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  4. Renata Silva Brandão
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  5. Rosália Santos Amorim Jesuino
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  6. Cirano José Ulhoa
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Correspondence to Cirano José Ulhoa.

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Qualhato, T.F., Lopes, F.A.C., Steindorff, A.S. et al. Mycoparasitism studies of Trichoderma species against three phytopathogenic fungi: evaluation of antagonism and hydrolytic enzyme production. Biotechnol Lett 35, 1461–1468 (2013). https://doi.org/10.1007/s10529-013-1225-3

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  • DOI: https://doi.org/10.1007/s10529-013-1225-3

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