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Mycoparasitism studies of Trichoderma harzianum strains against Rhizoctonia solani: evaluation of coiling and hydrolytic enzyme production

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Abstract

The genus Trichoderma is a potential biocontrol agent against several phytopathogenic fungi. One parameter for its successful use is an efficient coiling process followed by a substantial production of hydrolytic enzymes. The interaction between fifteen isolates of Trichoderma harzianum and the soil-borne plant pathogen, Rhizoctonia solani, was studied by light microscopy and transmission electron microscopy (TEM). Macroscopic observations of fungal growth in dual cultures revealed that growth inhibition of the pathogen occurred soon after contact with the antagonist. All T. harzianum isolates tested exhibited coiling around the hyphae of R. solani. The strains ALL23, ALL40, ALL41, ALL43 and ALL49 did not differ in coiling frequency and gave equal coiling performances. No correlation between coiling frequency and the production of cell wall-degrading chitinases, N-acetyl-β-d-glucosaminidase and β-1,3-glucanases, was found.

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References

  • Benitez T, Rincon AM, Limon MC, Codon AC (2004) Biocontrol mechanisms of Trichoderma strains. Int Microbiol 7(4):249–260

    PubMed  CAS  Google Scholar 

  • De Marco JL, Lima LHC, Valle de Souza M, Felix CR (2000) A Trichoderma harzianum chitinase destroys the cell wall of the phytopathogen Crinipellis perniciosa, the causal agent of witches broom disease of cocoa. World J Microbiol Biotecnol 16:383–386

    Article  Google Scholar 

  • El-Katany MH, Gudelj M, Robra K-H, Enaghy MA, Gubitz GM (2001) Characterization of a chitinase and an endo-1,3-glucanase from Trichoderma harzianum Rifae T24 involved in control of the phytopathogen Sclerotium rolfsii. Appl Microbiol Biotechnol 56:137–143

    Article  Google Scholar 

  • Howell CR (2003) Mechanisms employed by Trichoderma species in the biological control of plant diseases: the history and evolution of current concepts. Plant Dis 87:4–10

    Article  Google Scholar 

  • Innocenti G, Roberti R, Montanari M, Zakrissom E (2003) Efficacy of microorganisms antagonistic to Rhizoctonia cerealis and their cell degrading enzymatic activities. Mycol Res 107:421–427

    Article  PubMed  CAS  Google Scholar 

  • Kubicek CP, Mach RL, Peterbauer CK, Lorito M (2001) Trichoderma: from genes to biocontrol. J Plant Pathol 83:11–24

    CAS  Google Scholar 

  • Lima AL (2002) Molecular and biochemical characterization of Trichoderma isolated from Brazilian cerrado soil. PhD Thesis. Universidade de Brasília, Brasilia, Brazil

  • Mukherjee PK, Latha J, Hadar R, Horwitz BA (2003) TmkA, a mitogen-activated protein kinase of Trichoderma virens, is involved in biocontrol properties and repression of conidiation in the dark. Eukaryotic Cell 2(3):446–455

    Article  PubMed  CAS  Google Scholar 

  • Noronha EF, Ulhoa CJ (2000) Characterization of a 29-KDa β-1,3-glucanase from Trichoderma harzianum. FEMS Lett 183:119–123

    CAS  Google Scholar 

  • Omero C, Inbar J, Rocha-Ramirez V, Herrera-Estrela A, Chet I, Horwitz BA (1999) G proteins activators and cAMP promote mycoparasitic behavior in Trichoderma harzianum. Mycol Res 103(12):1637–1642

    Article  CAS  Google Scholar 

  • Silva RN , Silva SP, Brandão RL, Ulhoa CJ (2004) Regulation of N-acetyl-β-d-glucosaminidase produced by Trichoderma harzianum: evidence that cAMP controls its expression. Res Microbiol 155:667–671

    Article  PubMed  CAS  Google Scholar 

  • Ulhoa CJ, Peberdy JF (1992) Purification and some properties of the extracellular chitinase produced by Trichoderma harzianum. Enz Microbial Technol 14(3):236–240

    Article  CAS  Google Scholar 

  • Vazquez-Garciduenas S, Leal-Morales CA, Herrera-Estrella A (1998) Analysis of the b-1,3-glucanolytic system of the biocontrol agent Trichoderma harzianum. Appl Environ Microbiol 64(4):1442–1446

    PubMed  CAS  Google Scholar 

  • Zeilinger S, Galhaup C, Payer K, Woo SL, Mach RL, Fekete C, Lorito M, Kubicek CP (1999) Chitinase gene expression during mycoparasitic interaction of Trichoderma harzianum with its host. Fungal Genet Biol 26(2):131–140

    Article  PubMed  CAS  Google Scholar 

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Acknowledgements

This work was supported by a biotechnology research grant to C.J.U. (CNPq and FUNAPE/UFG). A.L.L. and R.N.S are supported by CAPES/UNB and F.B.R.A. and F.M.C are supported by PIBIC/CNPq. The authors thank Dr. Sônia Nair Baó (UnB/Cel) for her support with electron microscopy.

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Authors and Affiliations

  1. Laboratório de Enzimologia, Universidade Federal de Goiás (ICB), 74001-970, Goiânia, GO, Brazil

    Fausto Bruno dos Reis Almeida, Fernanda Menezes Cerqueira, Roberto do Nascimento Silva & Cirano José Ulhoa

  2. Embrapa Amapá, Rodovia Juscelino Kubitschek, Km 5, Caixa Postal 10, 68903-000, Macapá, AP, Brazil

    Adilson Lopes Lima

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  1. Fausto Bruno dos Reis Almeida
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  2. Fernanda Menezes Cerqueira
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  3. Roberto do Nascimento Silva
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  4. Cirano José Ulhoa
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  5. Adilson Lopes Lima
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Correspondence to Cirano José Ulhoa.

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Almeida, F.B.d.R., Cerqueira, F.M., Silva, R.d.N. et al. Mycoparasitism studies of Trichoderma harzianum strains against Rhizoctonia solani: evaluation of coiling and hydrolytic enzyme production. Biotechnol Lett 29, 1189–1193 (2007). https://doi.org/10.1007/s10529-007-9372-z

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  • DOI: https://doi.org/10.1007/s10529-007-9372-z

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