Abstract
A novel hydroxyapatite/regenerated silk fibroin scaffold was prepared and investigated for its potential to enhance both osteoinductivity and osteoconductivity of bone marrow-derived mesenchymal stromal cells in vitro. Approx. 12.4 ± 0.06 % (w/w) hydroxyapatite was deposited onto the scaffold, and cell viability and DNA content were significantly increased (18.5 ± 0.6 and 33 ± 1.2 %, respectively) compared with the hydroxyapatite scaffold after 14 days. Furthermore, alkaline phosphatase activity in the novel scaffold increased 41 ± 2.5 % after 14 days compared with the hydroxyapatite scaffold. The data indicate that this novel hydroxyapatite/regenerated silk fibroin scaffold has a positive effect on osteoinductivity and osteoconductivity, and may be useful for bone tissue engineering.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Altman GH, Diaz F, Jakuba C, Calabro T, Horan RL, Chen J, Lu H, Richmond J, Kaplan DL (2003) Silk-based biomaterials. Biomaterials 24(3):401–416
Bhumiratana S, Grayson WL, Castaneda A, Rockwood DN, Gil ES, Kaplan DL, Vunjak-Novakovic G (2011) Nucleation and growth of mineralized bone matrix on silk-hydroxyapatite composite scaffolds. Biomaterials 32(11):2812–2820
Cao Z, Chen X, Yao J, Huang L, Shao Z (2007) The preparation of regenerated silk fibroin microspheres. Soft Matter 3(7):910–915
Chen X, Knight DP, Shao Z (2009) [Small beta]-turn formation during the conformation transition in silk fibroin. Soft Matter 5(14):2777–2781
Fiedler J, Röderer G, Günther K-P, Brenner RE (2002) BMP-2, BMP-4, and PDGF-bb stimulate chemotactic migration of primary human mesenchymal progenitor cells. J Cell Biochem 87(3):305–312
Freed LE, Vunjak-Novakovic G, Biron RJ, Eagles DB, Lesnoy DC, Barlow SK, Langer R (1994) Biodegradable Polymer Scaffolds for Tissue Engineering. Nat Biotech 12(7):689–693
Jin H-J, Chen J, Karageorgiou V, Altman GH, Kaplan DL (2004) Human bone marrow stromal cell responses on electrospun silk fibroin mats. Biomaterials 25(6):1039–1047
Kim HJ, Kim U-J, Kim HS, Li C, Wada M, Leisk GG, Kaplan DL (2008) Bone tissue engineering with premineralized silk scaffolds. Bone 42(6):1226–1234
Na K, Sw Kim, Sun BK, Woo DG, Yang HN, Chung HM, Park KH (2007) Osteogenic differentiation of rabbit mesenchymal stem cells in thermo-reversible hydrogel constructs containing hydroxyapatite and bone morphogenic protein-2 (BMP-2). Biomaterials 28(16):2631–2637
Shao XX, Hutmacher DW, Ho ST, Goh JCH, Lee EH (2006) Evaluation of a hybrid scaffold/cell construct in repair of high-load-bearing osteochondral defects in rabbits. Biomaterials 27(7):1071–1080
Wang H, Li Y, Zuo Y, Li J, Ma S, Cheng L (2007) Biocompatibility and osteogenesis of biomimetic nano-hydroxyapatite/polyamide composite scaffolds for bone tissue engineering. Biomaterials 28(22):3338–3348
Zhou G, Shao Z, Knight DP, Yan J, Chen X (2009) Silk fibers extruded artificially from aqueous solutions of regenerated Bombyx mori silk fibroin are tougher than their natural counterparts. Adv Mater 21(3):366–370
Zhou J, Fang T, Wen J, Shao Z, Dong J (2011) Silk coating on poly(e-caprolactone) microspheres for the delayed release of vancomycin. J Microencapsul 28(2):99–107
Acknowledgments
This work was supported by the Grants from the Young Project of National Natural Science Foundation of China (81000816), 973 Project from the Ministry of Science and Technology of China (No. 2009CB930000), and the Project of Shanghai Municipal Science and Technology Commission (11JC1401700 and 12ZR1415800).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Jiang, J., Hao, W., Li, Y. et al. Hydroxyapatite/regenerated silk fibroin scaffold-enhanced osteoinductivity and osteoconductivity of bone marrow-derived mesenchymal stromal cells. Biotechnol Lett 35, 657–661 (2013). https://doi.org/10.1007/s10529-012-1121-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10529-012-1121-2