Abstract
Coupling lactate fermentation with cell growth was investigated in shake-flask and bioreactor cultivation systems by increasing aeration to improve lactate productivity in Escherichia coli CICIM B0013-070 (ackA pta pps pflB dld poxB adhE frdA). In shake-flasks, cells reached 1 g dry wt/l then, cultivated at 100 rpm and 42°C, achieved a twofold higher productivity of lactic acid compared to aerobic and O2-limited two-phase fermentation. The cells in the bioreactor yielded an overall volumetric productivity of 5.5 g/l h and a yield of 86 g lactic acid/100 g glucose which were 66% higher and the same level compared to that of the aerobic and O2-limited two-phase fermentation, respectively, using scaled-up conditions optimized from shake-flask experiments. These results have revealed an approach for improving production of fermentative products in E. coli.
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This work was partly funded by the Sino-South Africa Cooperation Program 2009DFA31300 and the National Natural Science Foundation of China No. 21006039.
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Li Zhou and Kang-Ming Tian have equal contribution.
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Zhou, L., Tian, KM., Niu, DD. et al. Improvement of d-lactate productivity in recombinant Escherichia coli by coupling production with growth. Biotechnol Lett 34, 1123–1130 (2012). https://doi.org/10.1007/s10529-012-0883-x
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DOI: https://doi.org/10.1007/s10529-012-0883-x