Abstract
A putative d-lyxose isomerase from Dictyoglomus turgidum was purified with a specific activity of 19 U/mg for d-lyxose isomerization by heat treatment and affinity chromatography. The native enzyme was estimated as a 42 kDa dimer by gel-filtration chromatography. The activity of the enzyme was highest for d-lyxose, suggesting that it is a d-lyxose isomerase. The maximum activity of the enzyme was at pH 7.5 and 75°C in the presence of 0.5 mM Co2+, with a half-life of 108 min, K m of 39 mM, and k cat of 3,570 1/min. The enzyme is the most thermostable d-lyxose isomerase among those characterized to date. It converted 500 g d-xylulose/l to 380 g d-lyxose/l after 2 h. This is the highest concentration and productivity of d-lyxose reported thus far.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Anderson RL, Allison DP (1965) Purification and characterization of d-lyxose isomerase. J Biol Chem 240:2367–2372
Bruce LZ, Henrik KN, Robert LS (1991) Thermostable enzymes for industrial applications. J Ind Microbiol Biotechnol 8:71–81
Brumm P, Hermanson S, Hochstein B, Boyum J, Hermersmann N, Gowda K, Mead D (2010) Mining Dictyoglomus turgidum for enzymatically active carbohydrases. Appl Biochem Biotechnol 163:205–214
Cho EA, Lee DW, Cha YH, Lee SJ, Jung HC, Pan JG, Pyun YR (2007) Characterization of a novel d-lyxose isomerase from Cohnella laevoribosii RI-39 sp. nov. J Bacteriol 189:1655–1663
Kwon HJ, Yeom SJ, Park CS, Oh DK (2010) Substrate specificity of a recombinant d-lyxose isomerase from Providencia stuartii for monosaccharides. J Biosci Bioeng 110:26–31
Morita M, Sawa E, Yamaji K, Sakai T, Natori T, Koezuka Y, Fukushima H, Akimoto K (1996) Practical total synthesis of (2S, 3S, 4R)-1-O-(α-d-galactopyranosyl)-N-hexacosanoyl-2-amino-1,3,4-octadecanetriol, the antitumorial and immunostimulatory α-galactosylcer-amide, KRN7000. Biosci Biotechnol Biochem 60:288–292
Park CS, Kwon HJ, Yeom SJ, Oh DK (2010a) Mannose production from fructose by free and immobilized d-lyxose isomerases from Providencia stuartii. Biotechnol Lett 32:1305–1309
Park CS, Yeom SJ, Lim YR, Kim YS, Oh DK (2010b) Substrate specificity of a recombinant d-lyxose isomerase from Serratia proteamaculans that produces d-lyxose and d-mannose. Lett Appl Microbiol 51:343–350
Patel DH, Wi SG, Lee SG, Lee DS, Song YH, Bae HJ (2011) Substrate specificity of the Bacillus licheniformis lyxose isomerase YdaE and its application in in vitro catalysis for bioproduction of lyxose and glucose by two-step isomerization. Appl Environ Microbiol 77:3343–3350
Takagi Y, Nakai K, Tsuchiya T, Takeuchi T (1996) A 5′-(trifluoromethyl)anthracycline glycoside: synthesis of antitumor-active 7-O-(2,6-dideoxy-6,6,6-trifluoro-alpha-l-lyxo-hexopyranosyl) adriamycinone. J Med Chem 39:1582–1588
van Staalduinen LM, Park CS, Yeom SJ, Adams-Cioaba MA, Oh DK, Jia Z (2010) Structure-based annotation of a novel sugar isomerase from the pathogenic E. coli O157:H7. J Mol Biol 401:866–881
Acknowledgment
This work was supported by Konkuk University in 2011.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Choi, JG., Hong, SH., Kim, YS. et al. Characterization of a recombinant thermostable d-lyxose isomerase from Dictyoglomus turgidum that produces d-lyxose from d-xylulose. Biotechnol Lett 34, 1079–1085 (2012). https://doi.org/10.1007/s10529-012-0874-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10529-012-0874-y