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Mannose production from fructose by free and immobilized d-lyxose isomerases from Providencia stuartii

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Abstract

A recombinant d-lyxose isomerase from Providencia stuartii was immobilized on Duolite A568 beads which gave the highest conversion of d-fructose to d-mannose among the various immobilization beads evaluated. Maximum activities of both the free and immobilized enzymes for fructose isomerization were at pH 7.5 and 45°C in the presence of 1 mM Mn2+. Enzyme half-lives were 14 and 30 h at 35°C and 3.4 and 5.1 h at 45°C, respectively. The immobilized enzyme in 300 g fructose/l (replaced hourly), produced 75 g mannose/l at 35°C = 25% (w/w) yield with a productivity of 75 g mannose l−1 h−1 after 23 cycles.

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Acknowledgments

This work was supported by the Korea Science and Engineering Foundation (KOSEF) through the National Research Lab. Program funded by the Ministry of Education, Science and Technology (R0A-2007-000-20015-0) and by a grant (Code #2007-0301034024) from BioGreen 21 Program, Rural Development Administration.

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  1. Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, South Korea

    Chang-Su Park, Hyun-Jung Kwon, Soo-Jin Yeom & Deok-Kun Oh

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  1. Chang-Su Park
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  2. Hyun-Jung Kwon
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  3. Soo-Jin Yeom
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  4. Deok-Kun Oh
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Correspondence to Deok-Kun Oh.

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Park, CS., Kwon, HJ., Yeom, SJ. et al. Mannose production from fructose by free and immobilized d-lyxose isomerases from Providencia stuartii . Biotechnol Lett 32, 1305–1309 (2010). https://doi.org/10.1007/s10529-010-0300-2

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  • DOI: https://doi.org/10.1007/s10529-010-0300-2

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