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De novo engineering and metabolic flux analysis of inosine biosynthesis in Bacillus subtilis

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Abstract

Wild-type B. subtilis strain W168 was de novo engineered for inosine biosynthesis. Inactivation of deoD and purA led to 0.15 ± 0.04 and 6.44 ± 0.39 g inosine/l yields, respectively. The deoD purA double mutant accumulated 7.6 ± 0.34 g inosine/l, with a 4.7% (w/w) conversion ratio from glucose to inosine. Comparative metabolic flux analysis revealed that the fluxes from inosine to hypoxanthine and from inosine monophosphate to adenosine monophosphate in the double mutant decreased to 14.0 and 0.61% of those in the wild-type strain. The major role of purA was demonstrated when inactivation of deoD and purA were found to contribute additively to inosine accumulation. This work is expected to contribute to the improvement of the fermentative production of purine nucleosides in the microbial industry.

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Acknowledgments

We are grateful to the Bacillus Genetic Stock Center (BGSC) and Prof. Ciarán Condon for providing the plasmids and strains used in this study. This research was funded by the National Drug Discovery Program of China (2008ZX09401-05), and Key Project of Chinese Academy of Sciences (KSCX2-EW-J-6).

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Authors and Affiliations

  1. College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China

    Haojian Li & Ning Chen

  2. Department of Industrial Microbiology and Biotechnology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China

    Guoqiang Zhang, Aihua Deng & Tingyi Wen

  3. Graduate University of Chinese Academy of Sciences, Beijing, 100049, China

    Guoqiang Zhang & Aihua Deng

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  1. Haojian Li
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  2. Guoqiang Zhang
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  3. Aihua Deng
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  4. Ning Chen
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Correspondence to Tingyi Wen.

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Haojian Li, Guoqiang Zhang: Co-first authors.

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Li, H., Zhang, G., Deng, A. et al. De novo engineering and metabolic flux analysis of inosine biosynthesis in Bacillus subtilis . Biotechnol Lett 33, 1575–1580 (2011). https://doi.org/10.1007/s10529-011-0597-5

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  • DOI: https://doi.org/10.1007/s10529-011-0597-5

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