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Overexpression of glucose-6-phosphate dehydrogenase enhances riboflavin production in Bacillus subtilis

  • Applied Genetics and Molecular Biotechnology
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Abstract

Carbon flow in Bacillus subtilis through the pentose phosphate (PP) pathway was modulated by overexpression of glucose-6-phosphate dehydrogenase (G6PDH) under the control of the inducible Pxyl promoter in B. subtilis PY. Alteration of carbon flow into the PP pathway will affect the availability of ribulose-5-phosphate (Ru5P) and the riboflavin yield. Overexpression of G6PDH resulted in the glucose consumption rate increasing slightly, while the specific growth rate was unchanged. An improvement by 25% ± 2 of the riboflavin production was obtained. Compared to by-products formation in flask culture, low acid production (acetate and pyruvate) and more acetoin were observed. Metabolic analysis, together with carbon flux redistribution, indicated that the PP pathway fluxes are increased in response to overexpression of G6PDH. Moreover, increased flux of the PP pathway is associated with an increased intracellular pool of Ru5P, which is a precursor for riboflavin biosynthesis. The high concentrations of Ru5P could explain the increased riboflavin production.

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Acknowledgments

The authors are grateful for the financial support from the National Natural Science Foundation of China (NSFC-20536040), the National Project of Key Fundamental Research (2007CB707802), and the Development Project of Science and Technology of Tianjin (05YFGZGX04500).

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  1. Yun Xia Duan

    Present address: Tianjin Academy of Environmental Sciences, Fukang Road, Tianjin, 300191, China

Authors and Affiliations

  1. Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, 92 Weijin Road, Tianjin, 300072, China

    Yun Xia Duan, Tao Chen, Xun Chen & Xue Ming Zhao

  2. Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin, 300072, China

    Yun Xia Duan, Tao Chen, Xun Chen & Xue Ming Zhao

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  1. Yun Xia Duan
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  2. Tao Chen
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Correspondence to Xue Ming Zhao.

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Duan, Y.X., Chen, T., Chen, X. et al. Overexpression of glucose-6-phosphate dehydrogenase enhances riboflavin production in Bacillus subtilis . Appl Microbiol Biotechnol 85, 1907–1914 (2010). https://doi.org/10.1007/s00253-009-2247-6

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  • DOI: https://doi.org/10.1007/s00253-009-2247-6

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