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High throughput determination of glucan and xylan fractions in lignocelluloses

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Abstract

The analysis of structural glucan and xylan in lignocellulose was scaled down from original two-stage sulfuric acid hydrolysis methods (Moore WE and Johnson DB 1967 Procedures for the chemical analysis of wood and wood products. U.S. Forest Products Laboratory, U.S. Department of Agriculture., Madison, WI) and integrated into a recently-developed, high throughput pretreatment and enzymatic saccharification system. Novel 96 × 1.8 ml-well Hastelloy reactor plates (128 × 86 × 51 mm) based on previously described 96-well pretreatment reactor plates were paired with custom aluminum filler plates (128 × 86 × 18 mm) for use in Symyx Powdernium solids dispensing systems. The incorporation of glucose oxidase and xylose dehydrogenase linked assays to speed post-hydrolysis sugar analysis dramatically reduced the time for analysis of large lignocellulosic sample sets. The current system permits the determination of the glucan and xylan content of 96 replicates (per reactor plate) in under 6 h and parallel plate processing increases the analysis throughput substantially.

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Acknowledgments

The BioEnergy Science Center is a U.S. Department of Energy Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science. The National Renewable Energy Laboratory is a national laboratory of the U.S. Department of Energy Office of Energy Efficiency and Renewable Energy Operated by the Alliance for Sustainable Energy, LLC.

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Authors and Affiliations

  1. Biosciences Center, National Renewable Energy Laboratory, Golden, CO, 80401, USA

    Michael J. Selig, Cody Law, Michael E. Himmel & Stephen R. Decker

  2. National Bioenergy Center, National Renewable Energy Laboratory, Golden, CO, 80401, USA

    Melvin P. Tucker & Crissa Doeppke

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  1. Michael J. Selig
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  2. Melvin P. Tucker
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  3. Cody Law
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  4. Crissa Doeppke
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  5. Michael E. Himmel
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  6. Stephen R. Decker
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Correspondence to Michael J. Selig.

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Selig, M.J., Tucker, M.P., Law, C. et al. High throughput determination of glucan and xylan fractions in lignocelluloses. Biotechnol Lett 33, 961–967 (2011). https://doi.org/10.1007/s10529-011-0526-7

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  • DOI: https://doi.org/10.1007/s10529-011-0526-7

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