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FT-IR imaging and pyrolysis-molecular beam mass spectrometry: new tools to investigate wood tissues

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Abstract

Fourier transform infrared (FT-IR) microimaging spectroscopy and pyrolysis molecular beam mass spectrometry (py-MBMS) were used as rapid analysis tools to evaluate differences in the chemical composition of 1-year-old transgenic aspens. Multivariate analysis of the spectroscopic data sets was used to compare the cell wall composition of nontransformed control to transgenic aspen plants with GRP-iaaM gene and with GRP-iaaM/35S-ACCase gene. Principal component analysis (PCA) was applied to both the FT-IR and py-MBMS spectra, which revealed sample groupings due to differences in chemical composition. Evaluating the PCA loadings allows determination of the chemical features responsible for the groupings. The FT-IR microimaging data was also used to monitor changes in the chemical composition as a function of the distance from the pith to the bark using partial least squares (PLS) analysis. The analysis shows that the changes in the composition of the xylem that occur over one annual growth ring can be monitored with FT-IR microimaging.

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Acknowledgements

This work was supported by the USDA, National Research Initiative Competitive Grant Program under contract number: 00-34158-9669 to T. Rials and the Consortium for Plant Biotechnology, Inc. by the Department of Energy Cooperative Agreement GO12026-155 to Y. Li and Z.M. Cheng. This support does not constitute an endorsement by DOE or by the Consortium for Plant Biotechnology Research, Inc. of the views expressed in this publication.

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Authors and Affiliations

  1. Tennessee Forest Products Center, University of Tennessee, 2506 Jacob Drive, Knoxville, TN, 37996-4570, USA

    Nicole Labbé & Timothy G. Rials

  2. National Renewable Energy Laboratory, National Bioenergy Center, 1617 Cole Blvd. , Golden, CO, 80401, USA

    Stephen S. Kelley

  3. Department of Plant Sciences, University of Tennessee, 2431 Joe Johnson Drive, Knoxville, TN, 37996, USA

    Zong-Ming Cheng & Joo-Young Kim

  4. Department of Plant Sciences, U-82, Transgenic Plant Facility, University of Connecticut, 105 Ahern Lane, Storrs, CT, 06269, USA

    Yi Li

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  1. Nicole Labbé
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  2. Timothy G. Rials
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  3. Stephen S. Kelley
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  4. Zong-Ming Cheng
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  5. Joo-Young Kim
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  6. Yi Li
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Correspondence to Nicole Labbé.

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Labbé, N., Rials, T.G., Kelley, S.S. et al. FT-IR imaging and pyrolysis-molecular beam mass spectrometry: new tools to investigate wood tissues. Wood Sci Technol 39, 61–76 (2005). https://doi.org/10.1007/s00226-004-0274-0

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