Abstract
A L-valine production strain Brevibacterium flavum C1(Ile−) was mutagenized using UV-irradiation and diethyl sulfate and was screened on the selective plates. The selective plates contained 4 g/L 2-thiazole-DL-alanine (2-TA) or 5 g/L α-aminobutyric acid (α-AB). After mutagenesis and selection, 1500 mutants were obtained, and 57 were found to be resistant to 2-thiazole-DL-alanine and α-aminobutyric acid. Following determination of the L-valine produced by these 57 mutants in fermentation medium in shaken cultures, a mutant CC5 (Ile−, 2-TAr, α-ABr) showed highest L-valine production ability than the original strain.
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The financial support of the Tianjin science and technology plan project (Science and technology innovation fund for small and medium-sized enterprises NO. 11ZXCXGX16300) is gratefully acknowledged.
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Cheng, K., Zhang, C. (2014). Screening, Mutagenesis of Brevibacterium flavum for the Enhancement of L-Valine Production. In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 249. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37916-1_29
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DOI: https://doi.org/10.1007/978-3-642-37916-1_29
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