Skip to main content
SpringerLink
Log in

Conversion of mannose to fructose by immobilized mannose isomerase fromPseudomonas cepacia

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

The enzyme mannose isomerase (EC 5.3.1.7) catalyzes the isomerization ofd-mannose andd-fructose. The conversion of mannose to fructose is the first step in the principal pathway of mannose dissimilation byPseudomonas cepacia. This enzyme is induced during growth on medium containing mannose to levels three- to four-fold higher than observed during growth on glucose or citrate. Mannose isomerase was purified from extracts of mannose-grownP. cepacia and was efficiently immobilized onto a porous, noncompressible, ceramic support. The performance of the immobilized enzyme, compared with the soluble enzyme, was evaluated under a variety of operating conditions to examine its potential for use in a process for the production of fructose syrups containing a higher proportion of fructose than is currently possible using glucose isomerase.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Palleroni, N. J. and Doudoroff, M. (1956),J. Biol. Chem. 218, 535.

    CAS  Google Scholar 

  2. Arias, A., Gardiol, A., and Martinez-Drets, G. (1982),J. Bacteriol. 151, 1069.

    CAS  Google Scholar 

  3. Takasaki, Y. (1967),Agr. Biol. Chem. 31, 435.

    CAS  Google Scholar 

  4. Hey-Ferguson, A. and Elbein, A. D. (1970),J. Bacteriol. 101, 777.

    CAS  Google Scholar 

  5. Thompson, N. S. (1981),Organic Chemicals from Biomass, Goldstein, I. S., ed., CRC Press, Boca Raton, FL, p. 125.

    Google Scholar 

  6. Herrick, F. W., Casebier, R. L., Hamilton, J. K., and Wilson, J. D. (1975),Appl. Polym. Symp. 28, 93.

    CAS  Google Scholar 

  7. Casebier, R. L., Herrick, F. W., Gray, K. R., Johnston, S., and Johnston, F. A. (1972),US Patent No. 3,677,818.

  8. Levy, J. and Fusee, M. C. (1979), USPatent No. 4,141,857.

  9. Roe, J. H. (1934),J. Biol. Chem. 107, 15.

    CAS  Google Scholar 

  10. Allenza, P., Scherl, D. S., and Detroy, R. W. (1986),Biotechnol. Bioeng. Symp. 17, 425.

    CAS  Google Scholar 

  11. Allenza, P. (1983),Ph.D. Dissertation, Univ. of Massachusetts, Amherst, pp. 69–75.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Allied- Signal Research and Technology, Des Plaines, IL

    P. Allenza, M. J. Morrell & R. W. Detroy

Authors
  1. P. Allenza
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. J. Morrell
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. W. Detroy
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Allenza, P., Morrell, M.J. & Detroy, R.W. Conversion of mannose to fructose by immobilized mannose isomerase fromPseudomonas cepacia . Appl Biochem Biotechnol 24, 171–182 (1990). https://doi.org/10.1007/BF02920243

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02920243

Index Entries

Search

Navigation