Abstract
Xyr1 (xylanase regulator 1) and ACEI are two transcriptional factors that regulate xylanases and cellulases formation in Trichoderma reesei. To investigate the transcriptional regulation of cellulases, DNA binding domains of both Xyr1 and ACEI in T. reesei were expressed from E. coli. Electrophoresis mobility shift assays showed that both formed complexes with the cbh1 promoter fragment (−304 to −18), suggesting that Xyr1 and ACEI may regulate cbh1 expression by bindings to the corresponding sites in the cbh1 promoter.
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Acknowledgements
This work was supported in part by grants from the National Natural Science Foundation of China (20066001) and the Open Fund of Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization (SB0709).
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Ling, M., Qin, Y., Li, N. et al. Binding of two transcriptional factors, Xyr1 and ACEI, in the promoter region of cellulase cbh1 gene. Biotechnol Lett 31, 227–231 (2009). https://doi.org/10.1007/s10529-008-9857-4
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DOI: https://doi.org/10.1007/s10529-008-9857-4