Abstract
In an attempt to clarify the function of lactose in cellulase induction, experiments were carried out on cellulase formation by lactose along with other sugars in a resting cell system of Trichoderma reesei PC-3-7, a hypercellulase-producing mutant. Although lactose alone induces little cellulase under the conditions used, a synergistic effect on cellulase formation was observed following the respective addition of sophorose, cellobiose or galactose to lactose. The lactose consumption was more rapid when these sugars were added than in their absence. Furthermore, following lactose addition 10 h after the beginning of cultivation in the presence of cellobiose, cellulase formation was initiated with only a little lag, and lactose consumption started immediately, being complete in 14 h. \-Galactosidase induction experiments suggested that the rapid consumption of lactose is possibly not dependent on lactose degradation by the enzyme. From these results, it is suggested that lactose may function as an inducer for cellulase formation if it is taken up in the mycelium of T. reesei PC-3-7, and that sophorose, cellobiose or galactose may induce a putative lactose permease. *** DIRECT SUPPORT *** AG903066 00005
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Chaudhuri BK, Sahai V (1993) Production of cellulase enzyme from lactose in batch and continuous cultures by a partially constitutive strain of Trichoderma reesei. Enzyme Microb Technol 15:513–518
Fritscher C, Messner R, Kubicek CP (1990) Cellobiose metabolism and cellobiohydrolase I biosynthesis by Trichoderma reesei. Exp Mycol 14:405–415
Fowler T, Brown RD Jr (1992) The bgl1 gene encoding extracellular \-glucosidase from Trichoderma reesei is required for rapid induction of the cellulase complex. Mol Microbiol 6:3225–3235
Hrmovà M, Biely P, Vrsanska M (1986) Specificity of cellulase and \-xylanase induction in Trichoderma reesei QM9414. Arch Microbiol 144:307–311
Iyayi CB, Bruchmann E-E, Kubicek CP (1989) Induction of cellulase formation in Trichoderma reesei by cellobiono-1,5-lactone. Arch Microbiol 151:326–330
Jung K, Jung H, Kaback HR (1994) Dynamics of lactose permease of Escherichia coli determined by site-directed fluorescence labeling. Biochemistry 33:3980–3985
Kawamori M, Morikawa Y, Takasawa S (1986a) Induction and production of cellulases by l-sorbose in Trichoderma reesei. Appl Microbiol Biotechnol 24:449–453
Kawamori M, Morikawa Y, Ado Y, Takasawa S (1986b) Production of cellulase from alkali-treated bagasse in Trichoderma reesei. Appl Microbiol Biotechnol 24:454–458
Kubicek CP (1992) The cellulase proteins of Trichoderma reesei: structure, multiplicity, mode of action and regulation of formation. Adv Biochem Eng Biotechnol 45:1–27
Kubicek CP, Messner R, Gruber F, Mandels M, Kubicek-Pranz EM (1993) Triggering of cellulase biosynthesis by cellulose in Trichoderma reesei. J Biol Chem 268:19364–19368
Kurosawa T, Yachi M, Suto M, Kamagata Y, Takao S, Tomita F (1992) Induction of cellulase by gentiobiose and its sulfur-containing analog in Penicilium purpurogenum. Appl Environ Microbiol 58:106–110
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Merivouri H, Siegler KM, Sands JA, Montenecourt BS (1984) Regulation of cellulase biosynthesis and secretion in fungi. Biochem Soc Trans 13:411–414
Messner R, Kubicek CP (1991) Carbon source control of cellobiohydrolase I and II formation by Trichoderma reesei. Appl Environ Microbiol 57:630–635
Messner R, Kubicek-Pranz EM, Gsur A, Kubicek CP (1991) Cellobiohydrolase II is the main conidial-bound cellulase in Trichoderma reesei and other Trichoderma strains. Arch Microbiol 155:601–606
Morikawa Y, Kawamori M, Ado Y, Shinsha Y, Oda F, Takasawa S (1985) Improvement of cellulase production in Trichoderma reesei. Agric Biol Chem 49:1869–1871
Nisizawa T, Suzuki H, Nisizawa K (1972) Catabolite repression of cellulase formation in Trichoderma viride. J Biochem (Tokyo) 71:999–1007
Pourquie J, Desmarquest J-P (1989) Scale up of cellulases production by Trichoderma reesei. In: Coughlan MP (ed) Enzyme systems for lignocellulose degradation. Elsevier, London, pp 283–292
Seiboth B, Messner R, Gruber F, Kubicek CP (1992) Disruption of the Trichoderma reesei cbh2 gene coding for cellobiohydrolase II leads to a delay in the triggering of cellulase formation by cellulose. J Gen Microbiol 138:1259–1264
Sternberg D, Mandels GR (1979) Induction of cellulolytic enzymes in Trichoderma reesei by sophorose. J Bacteriol 139:761–769
Strauss J, Kubicek CP (1990) \-Glucosidase and cellulase formation by a Trichoderma reesei mutant defective in constitutive \-glucosidase formation. J Gen Microbiol 136:1321–1326
Teeri TT, Penttila M, Keränen S, Nevalainen H, Knowles JKC (1992) Structure, function, and genetics of cellulases. In: Finkelstein DB, Ball C (eds) Biotechnology of filamentous fungi: technology and products. Butterworth-Heinemann, Boston, pp 417–445
Warzywoda M, Larbre E, Pourquie J (1992) Production and characterization of cellulolytic enzymes from Trichoderma reesei grown on various carbon sources. Bioresource Technol 39:125–130
Wood TM, Bhat KM (1988) Methods for measuring cellulase activities. Methods Enzymol 160:87–112
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Morikawa, Y., Ohashi, T., Mantani, O. et al. Cellulase induction by lactose in Trichoderma reesei PC-3-7. Appl Microbiol Biotechnol 44, 106–111 (1995). https://doi.org/10.1007/BF00164488
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00164488